Mellor J D, Hobkirk R
Can J Biochem. 1975 Jul;53(7):779-83. doi: 10.1139/o75-105.
17beta-[6,7-3H]Estradiol (E2) was incubated with slices and homogenates of adult human renal tissue. The metabolites formed were identified by chromatography on DEAE-Sephadex, thin layer chromatography and crystallization with carrier steroids or steroid derivatives. The major metabolites formed by slices were estradiol-17-glucuronide (E217G), estrone sulfate and estradiol-3-sulfate. This is the first report of in vitro synthesis of estrogen sulfates by adult renal tissue. Minor quantities of the 3-glucuronides of estrone and estradiol were also found. An oxygen atmosphere appeared to stimulate the production of E217G. A time study with tissue slices showed similarities between the in vitro pattern of glucuronide synthesis and the excretion pattern of these compounds seen in earlier in vivo studies. Homogenates fortified with uridine diphosphoglucuronic acid formed the same pattern of glucuronide products but in lesser amounts. No sulfates were formed under these conditions. Testosterone did not act as a substrate in the experimental conditions used.
将17β-[6,7-³H]雌二醇（E2）与成人肾脏组织切片及匀浆一起孵育。通过在二乙氨基乙基葡聚糖上进行色谱分析、薄层色谱分析以及与载体类固醇或类固醇衍生物结晶来鉴定所形成的代谢产物。切片形成的主要代谢产物为雌二醇-17-葡萄糖醛酸苷（E217G）、硫酸雌酮和硫酸雌二醇-3。这是关于成人肾脏组织体外合成雌激素硫酸盐的首次报道。还发现了少量的雌酮和雌二醇的3-葡萄糖醛酸苷。有氧环境似乎会刺激E217G的产生。对组织切片进行的时间研究表明，葡萄糖醛酸苷合成的体外模式与早期体内研究中观察到的这些化合物的排泄模式相似。用尿苷二磷酸葡萄糖醛酸强化的匀浆形成了相同模式的葡萄糖醛酸苷产物，但量较少。在这些条件下未形成硫酸盐。在所用的实验条件下，睾酮不作为底物。
