Chan A H, Robinson A R, Common R H
Steroids. 1975 May;25(5):677-95. doi: 10.1016/0039-128x(75)90022-7.
[4--14C] Estrone was injected intramuscularly into six laying hens. Fifty minutes later the hens were exsanguinated. The plasmas were examined for conjugates of radioactive phenolic steroids by recovery on columns of Amberlite XAD-2 or by extraction with tetrahydrofuran followed by chromatography on a column of DEAE-Sephadex A-25 in a gradient of NaCl. The biggest Sephadex chromatographic fraction (50,4% of total) contained about 42% of its radioactivity as estradiol-17alpha-3-sulfate and 18% as estradiol-17beta-3-sulfate and the remaining 40% was identified tentatively as estradiol-17alpha-17-sulfate plus a small proportion of estradiol-17beta-17-sulfate. The second biggest Sephadex chromatographic fraction (12.7% of total) was a mixture of conjugates not further identified. Minor fractions identified comprised estrone-beta-glucuronide (2.8%), estradiol-17alpha-3-beta-glucuronide (2.8%), estradiol-17beta-3-beta-glucuronide (2.3%) and estrone sulfate (6.0%). Evidence was obtained for the presence of small proportions of estradiol-17alpha disulfate and estradiol-17beta disulfate.
将[4--14C]雌酮肌肉注射到6只产蛋母鸡体内。50分钟后对母鸡放血。通过在Amberlite XAD - 2柱上回收或用四氢呋喃萃取，然后在DEAE - Sephadex A - 25柱上以氯化钠梯度进行色谱分析，检测血浆中放射性酚类甾体的结合物。最大的Sephadex色谱馏分（占总量的50.4%）含有约42%的放射性为雌二醇 - 17α - 3 - 硫酸盐和18%为雌二醇 - 17β - 3 - 硫酸盐，其余40%初步鉴定为雌二醇 - 17α - 17 - 硫酸盐加上一小部分雌二醇 - 17β - 17 - 硫酸盐。第二大的Sephadex色谱馏分（占总量的12.7%）是未进一步鉴定的结合物混合物。鉴定出的次要馏分包括雌酮 - β - 葡糖醛酸苷（2.8%）、雌二醇 - 17α - 3 - β - 葡糖醛酸苷（2.8%）、雌二醇 - 17β - 3 - β - 葡糖醛酸苷（2.3%）和雌酮硫酸盐（6.0%）。获得了存在少量雌二醇 - 17α二硫酸盐和雌二醇 - 17β二硫酸盐的证据。
