Li Yinhuan, Lu Jiuru
School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Luminescence. 2006 Jul-Aug;21(4):251-5. doi: 10.1002/bio.915.
A new flow injection chemiluminescence (CL) method has been developed for the determination of six beta-lactam antibiotics, including amoxicillin, cefadroxil, cefoperazone sodium, cefazolin sodium, cefradine and ceftriaxone sodium. When the antibiotic was injected into a stream of KMnO4 with alkaline luminol, a strong CL signal was produced. The method allows the measurements of 0.1-50.0 mg/L amoxicillin, 0.1-80.0 mg/L cefadroxil, 1.0-30.0 mg/L cefoperazone sodium, 1.0-30.0 mg/L cefazolin sodium, 3.0-50.0 mg/L cefradine and 3.0-50.0 mg/L ceftriaxone sodium. The detection limits are 0.05 mg/L for amoxycillin, 0.05 mg/L for cefadroxil, 0.4 mg/L for cefoperazonum sodium, 0.4 mg/L for cefazolin sodium, 0.8 mg/L for cefradine and 0.8 mg/L for ceftriaxone sodium. The relative standard deviations in 11 repeated measurements are 0.6%, 0.8%, 1.5%, 1.2%, 0.4% and 0.3% for 3.0 mg/L amoxicillin, 1.0 mg/L cefadroxil, 10.0 mg/L cefoperazone sodium, 10.0 mg/L cefazolin sodium, 10.0 mg/L cefradine and 10.0 mg/L ceftriaxone sodium, respectively. The method was successfully applied to the determination of amoxicillin in pharmaceutical preparations. A possible CL reaction mechanism is also discussed.
已开发出一种新的流动注射化学发光（CL）方法，用于测定六种β-内酰胺类抗生素，包括阿莫西林、头孢羟氨苄、头孢哌酮钠、头孢唑林钠、头孢拉定和头孢曲松钠。当将抗生素注入含有碱性鲁米诺的高锰酸钾流中时，会产生强烈的化学发光信号。该方法可测定0.1 - 50.0 mg/L的阿莫西林、0.1 - 80.0 mg/L的头孢羟氨苄、1.0 - 30.0 mg/L的头孢哌酮钠、1.0 - 30.0 mg/L的头孢唑林钠、3.0 - 50.0 mg/L的头孢拉定和3.0 - 50.0 mg/L的头孢曲松钠。阿莫西林的检测限为0.05 mg/L，头孢羟氨苄为0.05 mg/L，头孢哌酮钠为0.4 mg/L，头孢唑林钠为0.4 mg/L，头孢拉定为0.8 mg/L，头孢曲松钠为0.8 mg/L。对于3.0 mg/L的阿莫西林、1.0 mg/L的头孢羟氨苄、10.0 mg/L的头孢哌酮钠、10.0 mg/L的头孢唑林钠、10.0 mg/L的头孢拉定和10.0 mg/L的头孢曲松钠，11次重复测量的相对标准偏差分别为0.6%、0.8%、1.5%、1.2%、0.4%和0.3%。该方法已成功应用于药物制剂中阿莫西林的测定。还讨论了可能的化学发光反应机理。
