Yman Ingrid Malmheden, Eriksson Anders, Johansson M Annette, Hellenäs Karl-Erik
National Food Administration, PO Box 622, SE-751 26 Uppsala, Sweden.
J AOAC Int. 2006 May-Jun;89(3):856-61.
An optical biosensor was used to develop both direct and sandwich immunoassays for the detection of proteins from milk, egg, hazelnut, peanut, shellfish, and sesame in food samples. Affinity-purified polyclonal antibodies raised against the proteins were immobilized on the biosensor chip. Food samples were injected and the proteins that bound to the antibodies on the surface were detected by a shift in the resonance angle. By adding a second antibody in a sandwich assay, matrix effects could be overcome and the sensitivity and selectivity enhanced. Detection of allergen levels down to 1-12.5 microg/g in food samples was demonstrated for the various assays. Good agreement of results was also obtained from parallel analysis with alternative immunoassays, including rocket immunoelectrophoresis, enzyme immunoassay, and immunoblotting. The present study demonstrates that the sensitivity of the described biosensor technique is comparable to the most sensitive enzymed-linked immunosorbent assays.
一种光学生物传感器被用于开发直接免疫测定法和夹心免疫测定法，以检测食品样品中牛奶、鸡蛋、榛子、花生、贝类和芝麻中的蛋白质。针对这些蛋白质产生的亲和纯化多克隆抗体被固定在生物传感器芯片上。注入食品样品，通过共振角的变化检测与表面抗体结合的蛋白质。在夹心测定法中加入第二抗体，可以克服基质效应并提高灵敏度和选择性。各种测定法均证明能够检测食品样品中低至1-12.5微克/克的过敏原水平。与包括火箭免疫电泳、酶免疫测定和免疫印迹在内的其他免疫测定法进行平行分析，也获得了结果的良好一致性。本研究表明，所描述的生物传感器技术的灵敏度与最灵敏的酶联免疫吸附测定法相当。
