Zhou Yang, Gong Guo-zhong
Center for Liver Diseases, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Zhonghua Gan Zang Bing Za Zhi. 2006 Jun;14(6):414-7.
To investigate whether the nonstructural protein 5A (NS5A) encoded by the human hepatitis C virus RNA genome could affect the expression of the survivin gene.
HCV NS5A expression plasmid (pCNS5A) was transfected into HepG2 cells using a lipofectin reagent and the HCV NS5A protein was detected using immuno-histochemistry staining. Survivin mRNA was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) and the expression of survivin protein was detected using Western blot.
HCV NS5A protein was detected in the cytoplasm of the HepG2 cells transfected with pCNS5A, and the expressions of survivin mRNA and survivin protein were both up-regulated in the presence of the HCV NS5A protein.
HCV NS5A protein can stimulate survivin protein expression, and this might be the result of the strengthening of the survivin gene transcription.
研究人类丙型肝炎病毒RNA基因组编码的非结构蛋白5A（NS5A）是否会影响生存素基因的表达。
使用脂质体转染试剂将HCV NS5A表达质粒（pCNS5A）转染至HepG2细胞中，采用免疫组织化学染色检测HCV NS5A蛋白。通过逆转录聚合酶链反应（RT-PCR）评估生存素mRNA，并使用蛋白质免疫印迹法检测生存素蛋白的表达。
在用pCNS5A转染的HepG2细胞的细胞质中检测到HCV NS5A蛋白，并且在存在HCV NS5A蛋白的情况下，生存素mRNA和生存素蛋白的表达均上调。
HCV NS5A蛋白可刺激生存素蛋白表达，这可能是生存素基因转录增强的结果。
