Bouchut A, Sautiere P E, Coustau C, Mitta G
Parasitologie Fonctionnelle et Evolutive, UMR 5555, CNRS Université de Perpignan, 52 Ave Paul Alduy, 66 860 Perpignan Cedex, France.
Acta Trop. 2006 Jul;98(3):234-46. doi: 10.1016/j.actatropica.2006.05.007. Epub 2006 Jun 21.
As an approach to investigate the suspected involvement of cellular factors in Biomphalaria glabrata resistance/susceptibility to Echinostoma caproni, we compared protein patterns from hemocytes collected from susceptible and resistant snails. This proteomic approach revealed that twelve hemocytic proteins exhibited significant differences in their apparent abundance. The genes corresponding to five of them were characterized by a combination of mass spectrometry and molecular cloning. They encode an aldolase, an intermediate filament protein, a cytidine deaminase, the ribosomal protein P1 and the histone H4. Furthermore, we investigated their expression in parasite-exposed or -unexposed snails. These last experiments revealed changes in transcript levels corresponding to intermediate filament and histone H4 proteins post-infection.
作为一种研究细胞因子可能参与光滑双脐螺对卡氏棘口吸虫抗性/易感性的方法，我们比较了从易感和抗性蜗牛收集的血细胞的蛋白质模式。这种蛋白质组学方法显示，十二种血细胞蛋白的表观丰度存在显著差异。其中五种蛋白对应的基因通过质谱分析和分子克隆相结合的方法进行了表征。它们分别编码一种醛缩酶、一种中间丝蛋白、一种胞苷脱氨酶、核糖体蛋白P1和组蛋白H4。此外，我们研究了它们在暴露于寄生虫或未暴露于寄生虫的蜗牛中的表达。这些最新实验揭示了感染后与中间丝蛋白和组蛋白H4蛋白相对应的转录水平变化。
