Bouchut A, Coustau C, Gourbal B, Mitta G
Parasitologie Fonctionnelle et Evolutive, UMR 5555, CNRS Université de Perpignan Perpignan, France.
Parasitology. 2007 Apr;134(Pt 4):575-88. doi: 10.1017/S0031182006001673. Epub 2006 Nov 13.
In order to elucidate mechanisms underlying snail/echinostome compatibility, numerous molecular studies comparing transcripts and proteins of Biomphalaria glabrata susceptible or resistant to Echinostoma caproni were undertaken. These studies focused on plasma and haemocytes of the two strains and revealed that some transcripts and/or proteins were differentially expressed between strains. The aim of the present study was to develop a complementary transcriptomic approach by constructing subtractive libraries. This work revealed some candidate transcripts already identified in previous studies (calcium-binding proteins and glycolytic enzymes) as well as novel candidate transcripts that were differentially represented between strains of B. glabrata. Among these newly identified genes, we revealed several genes potentially involved in immune processes encoding proteases, protease inhibitors, a lectin, an aplysianin-like molecule, and cell adhesion molecules.
为了阐明钉螺/棘口吸虫相容性的潜在机制，开展了大量分子研究，比较了对卡氏棘口吸虫敏感或抗性的光滑双脐螺的转录本和蛋白质。这些研究聚焦于两个品系的血浆和血细胞，结果显示某些转录本和/或蛋白质在品系间存在差异表达。本研究的目的是通过构建消减文库开发一种互补的转录组学方法。这项工作揭示了一些在先前研究中已鉴定出的候选转录本（钙结合蛋白和糖酵解酶）以及光滑双脐螺品系间差异表达的新候选转录本。在这些新鉴定的基因中，我们发现了几个可能参与免疫过程的基因，它们编码蛋白酶、蛋白酶抑制剂、一种凝集素、一种海兔素样分子和细胞粘附分子。
