Moore C X, Cooper G J
Amylin Corporation, San Diego, CA 92121.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):1-9. doi: 10.1016/0006-291x(91)91325-7.
Amylin is a pancreatic islet beta-cell peptide hormone which modulates carbohydrate metabolism in skeletal muscle and liver, and could contribute to impaired insulin sensitivity in Type II diabetes. Here we report the first description of amylin secretion from isolated beta-cells. We measured amylin secretion from HIT T15 beta-cells exposed to glucose, arginine, glucagon, somatostatin, tolbutamide, glyburide, or metformin. With the exception of glucagon at concentrations above 1 microM, all compounds induced parallel, dose-dependent changes in secretion of amylin and insulin. We conclude that: 1) insulin and amylin are co-secreted from islet beta-cells; (2) nutrient secretagogues and peptide modulators exert direct effects on beta-cells to alter amylin and insulin secretion; (3) most modulators of islet beta-cell secretion alter amylin and insulin in parallel, but differential secretion can occur; and (4) HIT cell line is a useful model in which to study amylin metabolism.
胰淀素是一种胰岛β细胞肽类激素，可调节骨骼肌和肝脏中的碳水化合物代谢，并可能导致II型糖尿病患者胰岛素敏感性受损。在此，我们首次报道了分离的β细胞分泌胰淀素的情况。我们测量了暴露于葡萄糖、精氨酸、胰高血糖素、生长抑素、甲苯磺丁脲、格列本脲或二甲双胍的HIT T15β细胞分泌的胰淀素。除了浓度高于1微摩尔的胰高血糖素外，所有化合物均诱导胰淀素和胰岛素分泌发生平行的、剂量依赖性变化。我们得出以下结论：1）胰岛素和胰淀素从胰岛β细胞共同分泌；（2）营养促分泌剂和肽调节剂对β细胞产生直接作用，以改变胰淀素和胰岛素分泌；（3）大多数胰岛β细胞分泌调节剂会同时改变胰淀素和胰岛素，但也可能发生差异分泌；（4）HIT细胞系是研究胰淀素代谢的有用模型。
