Fehmann H C, Weber V, Göke R, Göke B, Eissele R, Arnold R
Department of Internal Medicine, Philipps-University of Marburg, Fed. Rep. Germany.
Biochem Biophys Res Commun. 1990 Mar 30;167(3):1102-8. doi: 10.1016/0006-291x(90)90636-2.
The effect of synthetic rat amylin (10,100,1000 pmol/l) on glucose (10 mmol/) and arginine (10 mmol/l) -stimulated islet hormone release from the isolated perfused rat pancreas and on amylase release from isolated pancreatic acini was investigated. Amylin stimulated the insulin release during the first (+76%) and the second secretion period (+42%) at 1 nmol/l. The first phase of the glucagon release was inhibited concentration dependently by amylin and completely suppressed during the second phase. Amylin diminished the somatostatin release in a concentration dependent manner. This effect was more pronounced at the first than the second secretion period (1 nmol amylin: 1 phase: -60%, 2.phase: -22%). Amylin was without any effect on basal and CCK stimulated amylase release from isolated rat pancreatic acini. Our data suggest amylin, a secretory product of pancreatic B-cells, as a peptide with approximately strong paracrine effects within the Langerhans islet. Therefore, amylin might be involved in the regulation of glucose homeostasis.
研究了合成大鼠胰淀素（10、100、1000 pmol/l）对葡萄糖（10 mmol/l）和精氨酸（10 mmol/l）刺激的离体灌注大鼠胰腺胰岛激素释放以及对离体胰腺腺泡淀粉酶释放的影响。在1 nmol/l时，胰淀素在第一个分泌期（增加76%）和第二个分泌期（增加42%）刺激胰岛素释放。胰淀素浓度依赖性地抑制胰高血糖素释放的第一阶段，并在第二阶段完全抑制。胰淀素以浓度依赖性方式减少生长抑素释放。这种作用在第一个分泌期比第二个分泌期更明显（1 nmol胰淀素：第一阶段：-60%，第二阶段：-22%）。胰淀素对离体大鼠胰腺腺泡的基础和CCK刺激的淀粉酶释放没有任何影响。我们的数据表明，胰淀素作为胰腺β细胞的分泌产物，是一种在胰岛内具有较强旁分泌作用的肽。因此，胰淀素可能参与葡萄糖稳态的调节。
