Elazar Z, Fuchs S
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
J Mol Neurosci. 1991;3(1):1-6. doi: 10.1007/BF02896843.
Orthovanadate (in the micromolar range) inhibits the high-affinity binding of the D2 dopamine receptor to specific agonists (apomorphine and N-propylnorapomorphine), while it does not affect the binding to D2 antagonists (spiperone and haloperidol). These effects of vanadate resemble those observed with guanine nucleotides or their analogs. However, in contrast to the guanine nucleotides, vanadate does not induce dissociation of the D2 dopamine receptor from its related G proteins, suggesting that vanadate and guanine nucleotides may exert their effect on the D2 dopamine receptor via different mechanisms. The effect of vanadate on agonist binding was shown to be ATP dependent and correlated with increased protein phosphorylation.
原钒酸盐（在微摩尔范围内）可抑制D2多巴胺受体与特定激动剂（阿扑吗啡和N-丙基去甲阿扑吗啡）的高亲和力结合，而不影响其与D2拮抗剂（螺哌隆和氟哌啶醇）的结合。钒酸盐的这些作用类似于用鸟嘌呤核苷酸或其类似物所观察到的作用。然而，与鸟嘌呤核苷酸不同的是，钒酸盐不会诱导D2多巴胺受体与其相关G蛋白解离，这表明钒酸盐和鸟嘌呤核苷酸可能通过不同机制对D2多巴胺受体发挥作用。已证明钒酸盐对激动剂结合的作用是ATP依赖性的，且与蛋白质磷酸化增加相关。
