Smith J B
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6162-6. doi: 10.1073/pnas.80.20.6162.
Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 microM are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3H]thymidine incorporation and DNA synthesis. In the absence of serum the effect of vanadium was greatly potentiated by insulin. Vanadium ions produced no more than additive increases in [3H]thymidine incorporation when combined with epidermal growth factor, cholera toxin, or phorbol 12-myristate 13-acetate. Both vanadium compounds stimulated ouabain-inhibitable 86Rb+ uptake, indicating that the vanadium ions increase, rather than inhibit, Na+/K+ pump activity in the intact cell. Neither vanadium compound had any effect on cellular cAMP under a variety of different conditions. The mitogenic effect of the vanadium compounds was similar to that of colchicine. Taxol, which stabilizes cytoplasmic microtubules, prevented the stimulation of DNA synthesis by vanadium.
硫酸氧钒和原钒酸钠在5至50微摩尔的浓度范围内，对瑞士小鼠3T3和3T6细胞的静止培养物具有促有丝分裂作用。这些化合物使血清对[3H]胸苷掺入和DNA合成的剂量反应发生了显著变化。在无血清的情况下，胰岛素极大地增强了钒的作用。当钒离子与表皮生长因子、霍乱毒素或佛波酯12-肉豆蔻酸酯13-乙酸酯联合使用时，[3H]胸苷掺入的增加不超过相加作用。两种钒化合物均刺激了哇巴因抑制的86Rb+摄取，表明钒离子增加而非抑制完整细胞中的Na+/K+泵活性。在各种不同条件下，两种钒化合物对细胞cAMP均无任何影响。钒化合物的促有丝分裂作用与秋水仙碱相似。稳定细胞质微管的紫杉醇可阻止钒对DNA合成的刺激。
