Gore Christopher J, Rodríguez Ferran A, Truijens Martin J, Townsend Nathan E, Stray-Gundersen James, Levine Benjamin D
Australian Institute of Sport, Canberra, Australia.
J Appl Physiol (1985). 2006 Nov;101(5):1386-93. doi: 10.1152/japplphysiol.00342.2006. Epub 2006 Jun 22.
This study tested the hypothesis that athletes exposed to 4 wk of intermittent hypobaric hypoxia exposure (3 h/day, 5 days/wk at 4,000-5,500 m) or double-blind placebo increase their red blood cell volume (RCV) and hemoglobin mass (Hbmass) secondary to an increase in erythropoietin (EPO). Twenty-three collegiate level athletes were measured before (Pre) and after (Post) the intervention for RCV via Evans blue (EB) dye and in duplicate for Hbmass using CO rebreathing. Hematological indexes including EPO, soluble transferrin receptor, and reticulocyte parameters were measured on 8-10 occasions spanning the intervention. The subjects were randomly divided among hypobaric hypoxia (Hypo, n = 11) and normoxic (Norm, n = 12) groups. Apart from doubling EPO concentration 3 h after hypoxia there was no increase in any of the measures for either Hypo or Norm groups. The mean change in RCV from Pre to Post for the Hypo group was 2.3% (95% confidence limits = -4.8 to 9.5%) and for the Norm group was -0.2% (-5.7 to 5.3%). The corresponding changes in Hbmass were 1.0% (-1.3 to 3.3%) for Hypo and -0.3% (-2.6 to 3.1%) for Norm. There was good agreement between blood volume (BV) from EB and CO: EB BV = 1.03 x CO BV + 142, r2 = 0.85, P < 0.0001. Overall, evidence from four independent techniques (RCV, Hbmass, reticulocyte parameters, and soluble transferrin receptor) suggests that intermittent hypobaric hypoxia exposure did not accelerate erythropoiesis despite the increase in serum EPO.
接受4周间歇性低压低氧暴露（每天3小时，每周5天，海拔4000 - 5500米）或双盲安慰剂的运动员，由于促红细胞生成素（EPO）增加，其红细胞体积（RCV）和血红蛋白量（Hbmass）会增加。23名大学水平的运动员在干预前（Pre）和干预后（Post）通过伊文思蓝（EB）染料测量RCV，并使用CO再呼吸法重复测量Hbmass。在干预期间的8 - 10个时间点测量包括EPO、可溶性转铁蛋白受体和网织红细胞参数在内的血液学指标。受试者被随机分为低压低氧组（Hypo，n = 11）和常氧组（Norm，n = 12）。除了低氧暴露3小时后EPO浓度翻倍外，Hypo组和Norm组的任何测量指标均未增加。Hypo组从Pre到Post的RCV平均变化为2.3%（95%置信区间 = -4.8至9.5%），Norm组为 -0.2%（-5.7至5.3%）。Hbmass的相应变化，Hypo组为1.0%（-1.3至3.3%），Norm组为 -0.3%（-2.6至3.1%）。EB法和CO法测量的血容量（BV）之间具有良好的一致性：EB BV = 1.03×CO BV + 142，r2 = 0.85，P < 0.0001。总体而言，来自四种独立技术（RCV、Hbmass、网织红细胞参数和可溶性转铁蛋白受体）的证据表明，尽管血清EPO增加，但间歇性低压低氧暴露并未加速红细胞生成。
