Rodríguez F A, Ventura J L, Casas M, Casas H, Pagés T, Rama R, Ricart A, Palacios L, Viscor G
Unitat d'Hipobària INEFC-UB, Institut Nacional d'Educació Fisica de Catalunya, Universitat de Barcelona, Spain.
Eur J Appl Physiol. 2000 Jun;82(3):170-7. doi: 10.1007/s004210050669.
This study aimed to determine whether brief hypoxic stimuli in a hypobaric chamber are able to elicit erythropoietin (EPO) secretion, and to effectively stimulate erythropoiesis in the short term. In two different experiments, a set of haematological, biochemical, haemorheological, aerobic performance, and medical tests were performed in two groups of healthy subjects. In the first experiment, the mean plasma concentration of EPO ([EPO]) increased from 8.7 to 13.5 mU.ml-1 (55.2%; P < 0.01) after 90 min of acute exposure at 540 hPa, and continued to rise until a peak was attained 3 h after the termination of hypoxia. In the second experiment, in which subjects were exposed to a simulated altitude of up to 5500 m (504 hPa) for 90 min, three times a week for 3 weeks, all haematological indicators of red cell mass increased significantly, reaching the highest mean values at the end of the programme or during the subsequent 2 weeks, including packed cell volume (from 42.5 to 45.1%; P < 0.01), red blood cell count (from 4.55 x 10(6) to 4.86 x 10(6).l-1; P < 0.01), reticulocytes (from 0.5 to 1.4%; P < 0.01), and haemoglobin concentration (from 14.3 to 16.2 g.dl-1; P < 0.01), without an increase in blood viscosity. Arterial blood oxygen saturation during hypoxia was improved (from 60% to 78%; P < 0.05). Our most relevant finding is the ability to effectively stimulate erythropoiesis through brief intermittent hypoxic stimuli (90 min), in a short period of time (3 weeks), leading to a lower arterial blood desaturation in hypoxia. The proposed mechanism for these haematological and functional adaptations is the repeated triggering effect of EPO production caused by the intermittent hypoxic stimuli.
本研究旨在确定低压舱内的短暂低氧刺激是否能够引发促红细胞生成素（EPO）分泌，并在短期内有效刺激红细胞生成。在两项不同的实验中，对两组健康受试者进行了一系列血液学、生化、血液流变学、有氧运动能力及医学测试。在第一个实验中，在540 hPa下急性暴露90分钟后，促红细胞生成素的平均血浆浓度（[EPO]）从8.7 mU.ml-1增至13.5 mU.ml-1（升高55.2%；P < 0.01），并持续上升，直至缺氧终止后3小时达到峰值。在第二个实验中，受试者暴露于高达5500米（504 hPa）的模拟海拔90分钟，每周3次，共3周，红细胞总量的所有血液学指标均显著增加，在实验结束时或随后2周内达到最高平均值，包括血细胞比容（从42.5%增至45.1%；P < 0.01）、红细胞计数（从4.55×10(6)增至4.86×10(6).l-1；P < 0.01）、网织红细胞（从0.5%增至1.4%；P < 0.01）及血红蛋白浓度（从14.3 g.dl-1增至16.2 g.dl-1；P < 0.01），而血液粘度未增加。缺氧期间动脉血氧饱和度得到改善（从60%增至78%；P < 0.05）。我们最相关的发现是，通过短暂间歇性低氧刺激（90分钟），能够在短时间内（3周）有效刺激红细胞生成，从而降低缺氧时的动脉血氧饱和度下降。这些血液学和功能适应性变化的推测机制是间歇性低氧刺激引起促红细胞生成素产生的反复触发效应。
