Babb J R, Davies J G, Ayliffe G A
Hospital Infection Research Laboratory, Dudley Road Hospital, Birmingham, UK.
J Hosp Infect. 1991 Jun;18 Suppl B:41-9. doi: 10.1016/0195-6701(91)90262-7.
A technique for assessing the immediate and prolonged efficacy of surgical scrubs and alcoholic hand rubs is described. A mean baseline count is obtained from all volunteers and logarithmic reductions in resident skin flora immediately after one or more applications, and after wearing gloves for 3 h, are measured. Loose-fitting surgical gloves are used for sampling resident flora. Preparations were applied using a standard technique for 2 min, apart from one test with 70% isopropanol (IPA) in which the application time was 30 s. Two studies are described, one of which compared four chlorhexidine scrubs, and the second 70% IPA, 7.5% povidone-iodine scrub, 2% triclosan cleanser and unmedicated bar soap. In spite of their constituent similarity, the four chlorhexidine scrubs varied considerably in efficacy and user acceptability. A 2 min application of 70% IPA was the most effective treatment, and gave log10 reductions of 1.65 for immediate and 1.58 for prolonged effect. This was marginally more effective than a 30 s application, but the difference was not significant. 'Hibiscrub' was the most effective aqueous formulation and gave reductions of 1.01 for immediate effect and 1.16 for prolonged effect. The test described could be used by reference centres and manufacturers to assess the efficacy of new and existing surgical hand disinfection formulations.
本文描述了一种评估手术洗手用品和酒精擦手液即时及长期效果的技术。从所有志愿者身上获取平均基线计数，并测量在一次或多次使用产品后，以及戴手套3小时后，常驻皮肤菌群的对数减少情况。使用宽松的手术手套对常驻菌群进行采样。除了一项使用70%异丙醇（IPA）的测试，其涂抹时间为30秒外，其他制剂均采用标准技术涂抹2分钟。本文描述了两项研究，其中一项比较了四种洗必泰手术洗手用品，另一项比较了70% IPA、7.5%聚维酮碘手术洗手用品、2%三氯生清洁剂和无药皂。尽管四种洗必泰手术洗手用品的成分相似，但其效果和用户接受度差异很大。70% IPA涂抹2分钟是最有效的治疗方法，即时效果的对数减少为1.65，长期效果的对数减少为1.58。这比涂抹30秒略有效，但差异不显著。“ Hibiscrub”是最有效的水性制剂，即时效果的减少为1.01，长期效果的减少为1.16。参考中心和制造商可使用本文所述的测试来评估新的和现有的手术手部消毒制剂的效果。
