Baquero F, Patrón C, Cantón R, Martínez Ferrer M
Department of Microbiology, Hospital Ramón y Cajal, Madrid, Spain.
J Hosp Infect. 1991 Jun;18 Suppl B:5-11. doi: 10.1016/0195-6701(91)90257-9.
Bacteria-laden skin squames, detached bacterial clumps and isolated bacteria floating in skin fluids are the major infective units in hand and skin transmission of microorganisms. These units have differing ability to colonize new surfaces and may have different susceptibility to antiseptics. MIC-MBC testing on isolated bacteria serves to confirm the expected susceptibility of particular isolates and is useful for surveillance of the evolution of antiseptic resistance; however, it is often unreliable in predicting the in-vivo effect. In-vitro tests aimed at duplicating natural conditions (including the effect of antiseptics on bacterial biofilms, or better, on the different infective units) are under evaluation. Meanwhile, tests involving natural skin surfaces, like the Story test, offer reproducible and useful data.
携带细菌的皮肤鳞屑、分离的细菌团块以及漂浮在皮肤液中的孤立细菌，是手部和皮肤微生物传播中的主要感染单位。这些单位在定殖新表面方面具有不同的能力，并且可能对抗菌剂有不同的敏感性。对分离出的细菌进行最低抑菌浓度-最低杀菌浓度测试，有助于确认特定分离株的预期敏感性，并且对于监测抗菌耐药性的演变很有用；然而，它在预测体内效果方面往往不可靠。旨在复制自然条件（包括抗菌剂对细菌生物膜的影响，或者更好的是对不同感染单位的影响）的体外测试正在评估中。同时，涉及天然皮肤表面的测试，如史特瑞测试，可提供可重复且有用的数据。
