Anaya-López José L, López-Meza Joel E, Baizabal-Aguirre Victor M, Cano-Camacho Horacio, Ochoa-Zarzosa Alejandra
Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
Biotechnol Lett. 2006 Jul;28(14):1101-8. doi: 10.1007/s10529-006-9060-4. Epub 2006 Jun 23.
Plant defensins are antimicrobial peptides that exhibit mainly antifungal activity against a broad range of plant fungal pathogens. However, their actions against Candida albicans have not been extensively studied. The mRNA for gamma-thionin, a defensin from Capsicum chinense, has been expressed in bovine endothelial cells. The conditioned medium of these cells showed antifungal activity on germ tube formation (60-70% of inhibition) and on the viability of C. albicans (70-80% of inhibition). Additionally, C. albicans was not able to penetrate transfected cells. Conditioned medium from these cells also inhibited the viability (80%) of the human tumor cell line, HeLa.
植物防御素是一类抗菌肽，主要对多种植物真菌病原体表现出抗真菌活性。然而，它们对白色念珠菌的作用尚未得到广泛研究。来自辣椒的一种防御素γ-硫堇的信使核糖核酸已在牛内皮细胞中表达。这些细胞的条件培养基对芽管形成（60 - 70%的抑制率）和白色念珠菌的活力（70 - 80%的抑制率）表现出抗真菌活性。此外，白色念珠菌无法穿透转染的细胞。这些细胞的条件培养基还抑制了人类肿瘤细胞系HeLa的活力（80%）。
