一种新型二价植物源口服乙肝和人类免疫缺陷病毒疫苗的免疫原性

Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses.

作者信息

Shchelkunov Sergei N, Salyaev Rurik K, Pozdnyakov Sergei G, Rekoslavskaya Natalia I, Nesterov Andrei E, Ryzhova Tatiana S, Sumtsova Valentina M, Pakova Natalia V, Mishutina Uliana O, Kopytina Tatiana V, Hammond Rosemarie W

机构信息

State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, 630559, Russia.

出版信息

Biotechnol Lett. 2006 Jul;28(13):959-67. doi: 10.1007/s10529-006-9028-4. Epub 2006 Jun 23.

DOI:10.1007/s10529-006-9028-4

PMID:16794774

Abstract

A synthetic chimeric gene, TBI-HBS, encoding the immunogenic ENV and GAG epitopes of human immunodeficiency virus (HIV-1) and the surface protein antigen (HBsAg) of hepatitis B virus (HBV), was expressed in tomato plants. Tomato fruits containing the TBI-HBS antigen were fed to experimental mice and, on days 14 and 28 post-feeding, high levels of HIV- and HBV-specific antibodies were present in the serum and feces of the test animals. Intraperitoneal injection of a DNA vaccine directing synthesis of the same TBI-HBsAg antigen boosted the antibody response to HIV in the blood serum; however, it had no effect on the high level of antibodies produced to HBV.

摘要

一种合成嵌合基因TBI-HBS，编码人类免疫缺陷病毒（HIV-1）的免疫原性ENV和GAG表位以及乙型肝炎病毒（HBV）的表面蛋白抗原（HBsAg），在番茄植株中得到表达。将含有TBI-HBS抗原的番茄果实喂给实验小鼠，在喂食后第14天和第28天，实验动物的血清和粪便中出现了高水平的HIV和HBV特异性抗体。腹腔注射一种指导合成相同TBI-HBsAg抗原的DNA疫苗增强了血清中对HIV的抗体反应；然而，它对产生的高水平HBV抗体没有影响。