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用于开发抗乳头瘤病毒疫苗的新型植物表达系统

The New Plant Expression System for the Development of Vaccines against Papillomaviruses.

作者信息

Salyaev R K, Rekoslavskaya N I, Stolbikov A S

机构信息

Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia.

Irkutsk Scientific Center, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia.

出版信息

Dokl Biochem Biophys. 2019 May;484(1):52-54. doi: 10.1134/S1607672919010150. Epub 2019 Apr 22.

DOI:10.1134/S1607672919010150
PMID:31012013
Abstract

To enhance the synthesis of antigenic envelope proteins L1 of high-grade papillomavirus types HPV16, HPV18, HPV31, and HPV45, the sequence of the gene encoding the cucumber mosaic virus replicase (RdRP CMV) was inserted into the genetic construct. This made it possible to increase the production of these antigenic proteins to 25-27 μg/mg total soluble protein.

摘要

为增强高危型乳头瘤病毒HPV16、HPV18、HPV31和HPV45抗原性包膜蛋白L1的合成，将编码黄瓜花叶病毒复制酶（RdRP CMV）的基因序列插入基因构建体中。这使得这些抗原性蛋白的产量提高到25 - 27微克/毫克总可溶性蛋白。

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本文引用的文献

1
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Dokl Biochem Biophys. 2016 May;468(1):187-9. doi: 10.1134/S1607672916030078. Epub 2016 Jul 15.
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Safety and immunogenicity of a plant-produced recombinant hemagglutinin-based influenza vaccine (HAI-05) derived from A/Indonesia/05/2005 (H5N1) influenza virus: a phase 1 randomized, double-blind, placebo-controlled, dose-escalation study in healthy adults.一种植物源性重组血凝素流感疫苗（HAI-05）的安全性和免疫原性，该疫苗源自 A/印度尼西亚/05/2005（H5N1）流感病毒：一项在健康成年人中进行的 1 期随机、双盲、安慰剂对照、剂量递增研究。
Viruses. 2012 Nov 19;4(11):3227-44. doi: 10.3390/v4113227.
3
Candidate mucosal vaccine against hepatitis B based on tomatoes transgenic for the preS2-S gene.基于转preS2-S基因番茄的候选乙型肝炎黏膜疫苗。
Dokl Biochem Biophys. 2012 Sep-Oct;446:257-9. doi: 10.1134/S1607672912050109. Epub 2012 Nov 7.
4
Characterization, genetic diversity, and evolutionary link of Cucumber mosaic virus strain New Delhi from India.印度新德里黄瓜花叶病毒株的特征、遗传多样性和进化关系。
Biochem Genet. 2011 Feb;49(1-2):25-38. doi: 10.1007/s10528-010-9382-8. Epub 2010 Oct 27.
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Study of the mucosal immune response duration in mice after administration of a candidate edible vaccine based on transgenic tomato plants carrying the TBI-HBS gene.基于携带TBI-HBS基因的转基因番茄植株的候选可食用疫苗给药后小鼠黏膜免疫反应持续时间的研究。
Dokl Biochem Biophys. 2009 Sep-Oct;428:232-4. doi: 10.1134/s1607672909050020.
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