用于开发抗乳头瘤病毒疫苗的新型植物表达系统
The New Plant Expression System for the Development of Vaccines against Papillomaviruses.
作者信息
Salyaev R K, Rekoslavskaya N I, Stolbikov A S
机构信息
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia.
Irkutsk Scientific Center, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia.
出版信息
Dokl Biochem Biophys. 2019 May;484(1):52-54. doi: 10.1134/S1607672919010150. Epub 2019 Apr 22.
To enhance the synthesis of antigenic envelope proteins L1 of high-grade papillomavirus types HPV16, HPV18, HPV31, and HPV45, the sequence of the gene encoding the cucumber mosaic virus replicase (RdRP CMV) was inserted into the genetic construct. This made it possible to increase the production of these antigenic proteins to 25-27 μg/mg total soluble protein.
为增强高危型乳头瘤病毒HPV16、HPV18、HPV31和HPV45抗原性包膜蛋白L1的合成，将编码黄瓜花叶病毒复制酶（RdRP CMV）的基因序列插入基因构建体中。这使得这些抗原性蛋白的产量提高到25 - 27微克/毫克总可溶性蛋白。
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