Patel Minal A, Katyare Surendra S
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390 002, India,
Mol Cell Biochem. 2006 Dec;293(1-2):193-201. doi: 10.1007/s11010-006-9242-3. Epub 2006 Jun 23.
Effects of treatment with DHEA (0.2 mg or 1.0 mg / kg body weight for 7 days) on oxidative energy metabolism on liver mitochondria from developing and young adult rats were examined. Treatment with DHEA resulted in a progressive dose-dependent increase in the liver weights of the developing animals without change in the body weight. In the young adult rats treatment with 1.0 mg DHEA showed increase only in the body weight. Treatment with DHEA stimulated state 3 and state 4 respiration rates in developing as well as young adult rats in dose-dependent manner with all the substrates used; magnitude of stimulation was age-dependent. In young adults the extent of simulation of state 3 respiration rates declined at higher dose (1.0 mg) of DHEA with glutamate and succinate as substrates. Stimulation of state 3 respiration rates was accompanied by increase in contents of cytochrome aa3, b and c + c1 and stimulation of ATPase and dehydrogenases activities in dose- and age-dependent manner.
研究了用脱氢表雄酮（DHEA，0.2毫克或1.0毫克/千克体重，持续7天）处理对发育中和年轻成年大鼠肝脏线粒体氧化能量代谢的影响。用DHEA处理导致发育中动物的肝脏重量呈剂量依赖性逐渐增加，而体重没有变化。在年轻成年大鼠中，用1.0毫克DHEA处理仅使体重增加。用DHEA处理以剂量依赖性方式刺激了发育中和年轻成年大鼠在使用所有底物时的状态3和状态4呼吸速率；刺激程度与年龄有关。在年轻成年大鼠中，以谷氨酸和琥珀酸为底物时，较高剂量（1.0毫克）的DHEA会使状态3呼吸速率的模拟程度下降。状态3呼吸速率的刺激伴随着细胞色素aa3、b和c + c1含量的增加以及ATP酶和脱氢酶活性以剂量和年龄依赖性方式的刺激。
