脱氢表雄酮和皮质酮对肝脏类固醇受体结合及线粒体呼吸作用影响的体外研究
In vitro studies on the effects of dehydroepiandrosterone and corticosterone on hepatic steroid receptor binding and mitochondrial respiration.
作者信息
McIntosh M K, Pan J S, Berdanier C D
机构信息
Department of Food, Nutrition, University of North Carolina, Greensboro 27412.
出版信息
Comp Biochem Physiol Comp Physiol. 1993 Jan;104(1):147-53. doi: 10.1016/0300-9629(93)90022-v.
PMID:8094655
Abstract
- The in vitro effects of dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEA-S) and related steroids on glucocorticoid corticosterone (GC) binding in hepatic cytosol and hepatic mitochondrial respiration were examined in male BHE/cdb and Sprague-Dawley (SD) rats. 2. The Kd and the IC50 for GC binding in SD rats were 5- and 2-fold higher, respectively, than in BHE/cdb rats. 3. Hepatic cytosol from BHE/cdb rats bound six times more [3H]-GC to receptors than that from SD rats. 4. The percentage displacement and Bmax of GC for its receptors were similar for the two strains of rats. 5. DHEA and DHEA-S did not displace GC from its receptors. 6. DHEA and related non-sulfated steroids decreased state 3 mitochondrial respiration, respiratory control and oxidative phosphorylation capacity in a dose-dependent manner with malate + pyruvate as substrate.
摘要
- 在雄性BHE/cdb和Sprague-Dawley（SD）大鼠中，检测了脱氢表雄酮（DHEA）、硫酸脱氢表雄酮（DHEA-S）及相关类固醇对肝细胞溶质中糖皮质激素皮质酮（GC）结合以及肝线粒体呼吸的体外作用。2. SD大鼠中GC结合的解离常数（Kd）和半数抑制浓度（IC50）分别比BHE/cdb大鼠高5倍和2倍。3. BHE/cdb大鼠的肝细胞溶质与受体结合的[3H]-GC比SD大鼠的多6倍。4. 两种品系大鼠中GC与其受体的置换百分比和最大结合容量（Bmax）相似。5. DHEA和DHEA-S不会从其受体上置换GC。6. 以苹果酸+丙酮酸为底物时，DHEA和相关的非硫酸化类固醇以剂量依赖方式降低线粒体呼吸状态3、呼吸控制和氧化磷酸化能力。
相似文献
1
In vitro studies on the effects of dehydroepiandrosterone and corticosterone on hepatic steroid receptor binding and mitochondrial respiration.脱氢表雄酮和皮质酮对肝脏类固醇受体结合及线粒体呼吸作用影响的体外研究
Comp Biochem Physiol Comp Physiol. 1993 Jan;104(1):147-53. doi: 10.1016/0300-9629(93)90022-v.
2
Further studies on the effects of dehydroepiandrosterone on hepatic metabolism in BHE rats.脱氢表雄酮对BHE大鼠肝脏代谢影响的进一步研究。
Proc Soc Exp Biol Med. 1989 Dec;192(3):242-7. doi: 10.3181/00379727-192-42992.
3
Short-term effects of dehydroepiandrosterone treatment in rats on mitochondrial respiration.脱氢表雄酮对大鼠线粒体呼吸的短期影响。
J Nutr. 1991 Feb;121(2):240-50. doi: 10.1093/jn/121.2.240.
4
In vitro and in vivo inhibitory effect of dehydroepiandrosterone on respiration.脱氢表雄酮对呼吸的体外和体内抑制作用。
Endocrinol Exp. 1978 Sep;12(3):165-9.
5
Dehydroepiandrosterone and related steroids inhibit mitochondrial respiration in vitro.
Int J Biochem. 1989;21(10):1103-7. doi: 10.1016/0020-711x(89)90050-5.
6
Treatment with dehydroepiandrosterone (DHEA) stimulates oxidative energy metabolism in the liver mitochondria from developing rats.用脱氢表雄酮（DHEA）治疗可刺激发育中大鼠肝脏线粒体的氧化能量代谢。
Mol Cell Biochem. 2006 Dec;293(1-2):193-201. doi: 10.1007/s11010-006-9242-3. Epub 2006 Jun 23.
7
Antiobesity effects of dehydroepiandrosterone are mediated by futile substrate cycling in hepatocytes of BHE/cdb rats.脱氢表雄酮的抗肥胖作用是由BHE/cdb大鼠肝细胞中的无效底物循环介导的。
J Nutr. 1991 Dec;121(12):2037-43. doi: 10.1093/jn/121.12.2037.
8
Opposing actions of dehydroepiandrosterone and corticosterone in rats.脱氢表雄酮和皮质酮在大鼠体内的相反作用。
Proc Soc Exp Biol Med. 1999 Jul;221(3):198-206. doi: 10.1046/j.1525-1373.1999.d01-77.x.
9
Differential effects of adrenalectomy and starvation-refeeding on hepatic lipogenic responses to dehydroepiandrosterone and glucocorticoid in BHE and Sprague-Dawley rats.
J Nutr. 1988 Aug;118(8):1011-7. doi: 10.1093/jn/118.8.1011.
10
Strain differences in the dose-response curves of adrenalectomized, starved-refed rats to dehydroepiandrosterone (DHEA).
Proc Soc Exp Biol Med. 1988 Feb;187(2):216-22. doi: 10.3181/00379727-187-42657.
引用本文的文献
1
Impaired oocyte quality induced by dehydroepiandrosterone is partially rescued by metformin treatment.脱氢表雄酮诱导的卵母细胞质量受损可通过二甲双胍治疗得到部分挽救。
PLoS One. 2015 Mar 26;10(3):e0122370. doi: 10.1371/journal.pone.0122370. eCollection 2015.
2
Hormonal regulation of longevity in mammals.哺乳动物寿命的激素调节。
Ageing Res Rev. 2007 May;6(1):28-45. doi: 10.1016/j.arr.2007.02.005. Epub 2007 Feb 20.
3
Treatment with dehydroepiandrosterone (DHEA) stimulates oxidative energy metabolism in the liver mitochondria from developing rats.用脱氢表雄酮（DHEA）治疗可刺激发育中大鼠肝脏线粒体的氧化能量代谢。
Mol Cell Biochem. 2006 Dec;293(1-2):193-201. doi: 10.1007/s11010-006-9242-3. Epub 2006 Jun 23.
Zotero 插件