Verschure Pernette J
Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, Kruislaan 318, 1098SM Amsterdam, The Netherlands.
J Cell Biochem. 2006 Sep 1;99(1):23-34. doi: 10.1002/jcb.20957.
The organization of the genome in the nucleus is related to its function. The functional compartmentalization of the genome is described at the nuclear, chromosomal, subchromosomal, nucleosomal, and DNA sequence level. These descriptions originate from the techniques that were used for analysis. The different levels of compartmentalization are not easily reconciled, because the techniques applied to identify genome compartmentalization generally cannot be performed in combination. We have obtained a large body of information on individual "actors" and "scenes" in the nucleus regarding genome compartmentalization, but we still do not understand how and by what pieces of equipment the "actors" play their game. The next challenge is to understand the combined operation of the various levels of functional genome organization in the nucleus, that is, how do the epigenetic and genetic levels act together. In this paper, I will highlight some of the general features and observations of functional organization of the eukaryotic genome in interphase nuclei and discuss the concepts and views based on observed correlations between genome organization and function. I will reflect on what is to be expected from this field of research when the functional levels of genome compartmentalization are integrated. In this context I will draw attention to what might be needed to improve our understanding.
基因组在细胞核中的组织方式与其功能相关。基因组的功能分区在细胞核、染色体、亚染色体、核小体和DNA序列水平上进行描述。这些描述源自用于分析的技术。不同层次的分区并不容易协调一致，因为用于识别基因组分区的技术通常无法联合进行。我们已经获得了大量关于细胞核中基因组分区的各个“参与者”和“场景”的信息，但我们仍然不明白“参与者”是如何以及通过何种“设备”来进行“活动”的。下一个挑战是了解细胞核中功能基因组组织各层次的联合运作，也就是说，表观遗传和遗传水平是如何共同作用的。在本文中，我将重点介绍间期细胞核中真核基因组功能组织的一些一般特征和观察结果，并基于基因组组织与功能之间观察到的相关性来讨论相关概念和观点。我将思考当基因组分区的功能层次整合后，该研究领域可能会有哪些预期成果。在此背景下，我将提请注意为增进我们的理解可能需要做些什么。
