Department of Obstetrics and Gynecology, Wayne State University, 253 C. S. Mott Center, 275 E. Hancock St., Detroit, MI 48201, USA.
Placenta. 2010 May;31(5):365-72. doi: 10.1016/j.placenta.2010.02.012. Epub 2010 Mar 25.
Abnormal trophoblast invasion is associated with the most common and most severe complications of human pregnancy. The biology of invasion, as well as the etiology of abnormal invasion remains poorly understood. The aim of this study was to characterize the transcriptome of the HTR-8/SVneo human cytotrophoblast cell line which displays well characterized invasive and non-invasive behavior, and to correlate the activity of the transcriptome with nuclear matrix attachment and cell phenotype. Comparison of the invasive to non-invasive HTR transcriptomes was unremarkable. In contrast, comparison of the MARs on chromosomes 14-18 revealed an increased number of MARs associated with the invasive phenotype. These attachment areas were more likely to be associated with silent rather than actively transcribed genes. This study supports the view that nuclear matrix attachment may play an important role in cytotrophoblast invasion by ensuring specific silencing that facilitates invasion.
异常的滋养层细胞侵袭与人类妊娠最常见和最严重的并发症有关。侵袭的生物学特性以及异常侵袭的病因仍知之甚少。本研究的目的是描述 HTR-8/SVneo 人细胞滋养层细胞系的转录组特征，该细胞系表现出良好特征的侵袭和非侵袭行为，并将转录组的活性与核基质附着和细胞表型相关联。侵袭性与非侵袭性 HTR 转录组的比较无明显差异。相比之下，对染色体 14-18 上的 MARs 的比较显示，与侵袭表型相关的 MARs 数量增加。这些附着区域更可能与沉默而非活跃转录的基因相关联。本研究支持核基质附着可能通过确保特定的沉默来促进侵袭，从而在滋养层细胞侵袭中发挥重要作用的观点。
