Solvason N, Lehuen A, Kearney J F
Department of Microbiology, University of Alabama, Birmingham 35294.
Int Immunol. 1991 Jun;3(6):543-50. doi: 10.1093/intimm/3.6.543.
Ly1+ B cells differ from conventional B cells with respect to their anatomical localization, cell surface marker expression, and antibody repertoire suggesting that they may constitute a functionally distinct subset of B cells. To determine whether Ly1+ B cells also have a developmentally distinct site of origin we grafted various fetal primordia into adult severe combined immunodeficient (scid) mice and analyzed their potential to give rise to T and B cells. We demonstrated that fetal omentum, but not spleen or thymus grafts, reconstituted exclusively Ly1 B cells (including the Ly1 sister population) as well as a population of IgM and IgA producing plasma cells in the spleen and gut, respectively. Although thymus grafts regularly reconstituted T cells, thymus plus fetal omentum cografts gave rise to a population of Ly1+ B cells as well as T cells which were also derived from omentum. However, in neither omentum nor omentum plus thymus cografts were conventional B cells detected. These results provide the first evidence that Ly1 B cells but not conventional B cells are generated from the fetal omentum.
Ly1⁺ B细胞在解剖定位、细胞表面标志物表达和抗体库方面与传统B细胞不同，这表明它们可能构成B细胞的一个功能独特的亚群。为了确定Ly1⁺ B细胞是否也有发育上不同的起源部位，我们将各种胎儿原基移植到成年重症联合免疫缺陷（scid）小鼠体内，并分析它们产生T细胞和B细胞的潜力。我们证明，胎儿网膜移植而非脾脏或胸腺移植，分别在脾脏和肠道中专门重建了Ly1 B细胞（包括Ly1姐妹群体）以及产生IgM和IgA的浆细胞群体。尽管胸腺移植经常重建T细胞，但胸腺加胎儿网膜共移植产生了一群Ly1⁺ B细胞以及同样来源于网膜的T细胞。然而，在网膜移植或网膜加胸腺共移植中均未检测到传统B细胞。这些结果提供了首个证据，即Ly1 B细胞而非传统B细胞是由胎儿网膜产生的。
