EA 4666 Lymphocyte Normal - Pathologique et Cancers, HEMATIM, Université de Picardie Jules Verne, Amiens, France.
Laboratoire d'Hématologie, Centre Hospitalier Universitaire Amiens-Picardie, Amiens, France.
Front Immunol. 2018 Apr 4;9:683. doi: 10.3389/fimmu.2018.00683. eCollection 2018.
Chronic lymphocytic leukemia (CLL) is characterized by the clonal expansion of small mature-looking CD19+ CD23+ CD5+ B-cells that accumulate in the blood, bone marrow, and lymphoid organs. To date, no consensus has been reached concerning the normal cellular counterpart of CLL B-cells and several B-cell types have been proposed. CLL B-cells have remarkable phenotypic and gene expression profile homogeneity. In recent years, the molecular and cellular biology of CLL has been enriched by seminal insights that are leading to a better understanding of the natural history of the disease. Immunophenotypic and molecular approaches (including immunoglobulin heavy-chain variable gene mutational status, transcriptional and epigenetic profiling) comparing the normal B-cell subset and CLL B-cells provide some new insights into the normal cellular counterpart. Functional characteristics (including activation requirements and propensity for plasma cell differentiation) of CLL B-cells have now been investigated for 50 years. B-cell subsets differ substantially in terms of their functional features. Analysis of shared functional characteristics may reveal similarities between normal B-cell subsets and CLL B-cells, allowing speculative assignment of a normal cellular counterpart for CLL B-cells. In this review, we summarize current data regarding peripheral B-cell differentiation and human B-cell subsets and suggest possibilities for a normal cellular counterpart based on the functional characteristics of CLL B-cells. However, a definitive normal cellular counterpart cannot be attributed on the basis of the available data. We discuss the functional characteristics required for a cell to be logically considered to be the normal counterpart of CLL B-cells.
慢性淋巴细胞白血病（CLL）的特征是小成熟样 CD19+ CD23+ CD5+ B 细胞的克隆性扩张，这些细胞在血液、骨髓和淋巴器官中积累。迄今为止，关于 CLL B 细胞的正常细胞对应物尚未达成共识，已经提出了几种 B 细胞类型。CLL B 细胞具有显著的表型和基因表达谱同质性。近年来，CLL 的分子和细胞生物学因一些重要的见解而得到丰富，这些见解使人们对该疾病的自然史有了更好的理解。免疫表型和分子方法（包括免疫球蛋白重链可变基因突变状态、转录和表观遗传分析）比较正常 B 细胞亚群和 CLL B 细胞，为正常细胞对应物提供了一些新的见解。CLL B 细胞的功能特征（包括激活要求和向浆细胞分化的倾向）已经研究了 50 年。B 细胞亚群在功能特征方面存在很大差异。分析共同的功能特征可能揭示正常 B 细胞亚群和 CLL B 细胞之间的相似性，从而可以推测 CLL B 细胞的正常细胞对应物。在这篇综述中，我们总结了关于外周 B 细胞分化和人类 B 细胞亚群的最新数据，并根据 CLL B 细胞的功能特征提出了正常细胞对应物的可能性。然而，根据现有数据，不能明确地将正常细胞对应物归因于 CLL B 细胞。我们讨论了一个细胞被逻辑上认为是 CLL B 细胞的正常对应物所需的功能特征。
