普伐他汀对醋酸诱导的大鼠结肠炎的治疗作用及相关机制：大鼠实验

[Therapeutic effects of pravastatin on colitis induced by acetic acid and relevant mechanism: experiment with rats].

作者信息

Chen Chi, Ran Zhi-hua, Xiao Shu-dong

机构信息

Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2006 May 16;86(18):1284-8.

PMID:16796891

Abstract

OBJECTIVE

To investigate the therapeutic effects of pravastatin on colitis induced by acetic acid and the relevant mechanism.

METHODS

8% solution of acetic acid was infusion into the rectum of 50 male SD rats so as to establish models of ulcerative colitis induced by acetic acid and 10 rats were used as normal controls treated with regular feeding. Then the 50 models of ulcerative colitis were randomly assigned into 3 groups: model + placebo group (n = 20, infused intragastrically with normal saline 2 ml/d for 7 days), model + pravastatin group (n = 15, infused intragastrically with pravastatin 1 mg x kg(-1) x d(-1)), and model + salicylazosulfapyridium (SASP) group (n = 15, infused intragastrically with SASP 0.25 g x kg(-1) x d(-1)). The general conditions, food intake, weight, stool consistencies, stool bleeding of rats were observed then the disease activity index (DAI) and the therapeutic effects were evaluated. All the rats were sacrificed at day 8 with their colons taken out. The colon length, colon mucosa damage index (CMDI), and histological score were evaluated and nitric oxide (NO), malondialdehyde (MDA), and superoxide dismutase (SOD) of the tissue were measured as well.

RESULTS

Compared with those of the model + placebo and model + SASP groups, the colon length, DAI, CMDI, and histological score of the model + pravastatin group were significantly improved (all P < 0.05), the levels of NO and MDA were significantly reduced, while the SOD level was increased remarkably (both P < 0.05).

CONCLUSION

Pravastatin may ameliorate the colitis by antioxidant activity. Its therapeutic effect is more obvious than the conventional medicine SASP.

摘要

目的

探讨普伐他汀对乙酸诱导的结肠炎的治疗作用及其相关机制。

方法

将8%的乙酸溶液注入50只雄性SD大鼠直肠，以建立乙酸诱导的溃疡性结肠炎模型，10只大鼠作为正常对照，常规饲养。然后将50只溃疡性结肠炎模型随机分为3组：模型+安慰剂组（n = 20，每日灌胃2 ml生理盐水，共7天）、模型+普伐他汀组（n = 15，每日灌胃普伐他汀1 mg·kg⁻¹·d⁻¹）、模型+柳氮磺胺吡啶（SASP）组（n = 15，每日灌胃SASP 0.25 g·kg⁻¹·d⁻¹）。观察大鼠的一般情况、进食量、体重、粪便性状、粪便出血情况，然后评估疾病活动指数（DAI）和治疗效果。所有大鼠在第8天处死，取出结肠。评估结肠长度、结肠黏膜损伤指数（CMDI）和组织学评分，并检测组织中的一氧化氮（NO）、丙二醛（MDA）和超氧化物歧化酶（SOD）水平。