Yin Liyan, Huang Jiaquan, Li Wei, Liu Yongding
Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan 430074, China.
Toxicon. 2006 Aug;48(2):204-10. doi: 10.1016/j.toxicon.2006.05.002. Epub 2006 May 19.
Our previous studies showed that microcystin-RR could induce oxidative damage in plant cells as they do with animal cells. However, whether microcystin can induce plant cell apoptosis is still unknown. In this study, the morphological changes of tobacco BY-2 suspension cells exposed to microcystin-RR were observed under light microscopy and transmission electron microscopy, and apoptosis was clearly distinguished by intense perinuclear chromatin margination, condensation of nuclear chromatin after 6d exposure of 50mg/L (about 50microM) microcystin-RR. We also found that microcystin-RR can induce tobacco cell apoptosis in a dose- and time-dependent manner with flow cytometry analysis. Our study provides the first evidence that microcystins can induce plant cell apoptosis.
我们之前的研究表明，微囊藻毒素-RR能够像在动物细胞中那样诱导植物细胞产生氧化损伤。然而，微囊藻毒素是否能诱导植物细胞凋亡仍是未知的。在本研究中，利用光学显微镜和透射电子显微镜观察了暴露于微囊藻毒素-RR的烟草BY-2悬浮细胞的形态变化，在暴露于50mg/L（约50微摩尔）微囊藻毒素-RR 6天后，通过强烈的核周染色质边缘化、核染色质凝聚清晰地鉴别出凋亡。我们还通过流式细胞术分析发现，微囊藻毒素-RR能够以剂量和时间依赖的方式诱导烟草细胞凋亡。我们的研究首次证明微囊藻毒素能够诱导植物细胞凋亡。
