Ramos Humberto J O, Souza Emanuel M, Soares-Ramos Juliana R L, Pedrosa Fábio O
Department of Biochemistry and Molecular Biology, UFPR, C. Postal 19046, CEP 81531-990 Curitiba-PR, Brazil.
J Biotechnol. 2006 Nov 10;126(3):291-4. doi: 10.1016/j.jbiotec.2006.04.020. Epub 2006 Jun 23.
A modified antibiosis assay was used to evaluate growth inhibition of symbiotic and endophytic bacteria by E. coli strains producing Bacillus amyloliquefaciens ribonuclease, barnase. Inhibition zones were only observed when the assays were performed in minimal medium agar. However, bacterial growth inhibition was not detected when using rich medium or susceptible strains expressing the ribonuclease inhibitor protein, barstar. Our results suggest that barnase may act as a broad range bacteriocin. The ecological significance of these results is discussed.
采用改良的抗菌试验来评估产生解淀粉芽孢杆菌核糖核酸酶（巴那斯酶）的大肠杆菌菌株对共生菌和内生细菌的生长抑制作用。仅当在基本培养基琼脂中进行试验时才观察到抑菌圈。然而，使用丰富培养基或表达核糖核酸酶抑制剂蛋白（巴司星）的敏感菌株时，未检测到细菌生长抑制。我们的结果表明，巴那斯酶可能作为一种广谱细菌素。讨论了这些结果的生态学意义。
