嗜热细菌中的（ADP-核糖基）化反应。

The (ADP-ribosyl)ation reaction in thermophilic bacteria.

作者信息

Faraone-Mennella Maria Rosaria, De Maio Anna, Petrella Anna, Romano Maria, Favaloro Patrizia, Gambacorta Agata, Lama Licia, Nicolaus Barbara, Farina Benedetta

机构信息

Dipartimento di Biologia Strutturale e Funzionale, Facolta' di Scienze M.F.N., Universita Federico II, Via Cynthia, 80126 Napoli, Italy.

出版信息

Res Microbiol. 2006 Jul-Aug;157(6):531-7. doi: 10.1016/j.resmic.2006.01.004. Epub 2006 Feb 17.

DOI:10.1016/j.resmic.2006.01.004

PMID:16797930

Abstract

Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cells at different growth phases, and evidence of an active system was obtained in all analyzed microorganisms, with comparable enzymatic levels. Immunochemical analyses with polyclonal antibodies against both eukaryotic anti-(ADP-ribose) transferase and anti-poly(ADP-ribose) polymerase revealed, for all tested organisms, an immunosignal localized in the range of molecular masses between 43-53 kD. Several proteins of various molecular masses were found as ADP-ribose acceptors. Reaction product analyses showed mono(ADP-ribose) to be the only synthesized compound.

摘要

为了研究（ADP-核糖基）化系统的可能存在情况，选择了 Alicyclobacillus、Bacillus 和 Thermus 属的细菌。这些细菌是嗜热、需氧的，并且是从不同的地热来源分离出来的。在不同生长阶段的细胞中测试了（ADP-核糖基）化蛋白的活性和表达，并且在所有分析的微生物中都获得了活性系统的证据，酶水平相当。用针对真核抗（ADP-核糖）转移酶和抗聚（ADP-核糖）聚合酶的多克隆抗体进行的免疫化学分析表明，对于所有测试的生物体，免疫信号定位在 43 - 53 kD 的分子量范围内。发现了几种不同分子量的蛋白质作为 ADP-核糖受体。反应产物分析表明单（ADP-核糖）是唯一合成的化合物。