Ehrman J D, Bender E T, Stojilovic N, Sullivan T, Ramsier R D, Buczynski B W, Kory M M, Steiner R P
Department of Physics, The University of Akron, Akron, OH 44325, United States.
Colloids Surf B Biointerfaces. 2006 Jul 1;50(2):152-9. doi: 10.1016/j.colsurfb.2006.04.010. Epub 2006 May 5.
We present data and analyses concerning the adhesion of clinically relevant Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa (bacteria) and Candida albicans (yeast) to Zircaloy-2 (Zry-2) and Zircadyne-705 (Zr705) surfaces. These zirconium-based materials are similar to those now being used in total hip and knee replacements. Here we study clinical strains of microbes under shaken and stationary exposure conditions, and their ability to adhere to Zr surfaces having different oxide thicknesses. We use X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), viable counts, endotoxin assays, and statistical analysis methods, and demonstrate a predictive model for microbial adhesion based on XPS data.
我们展示了关于临床相关的金黄色葡萄球菌、表皮葡萄球菌、铜绿假单胞菌（细菌）和白色念珠菌（酵母）与锆合金-2（Zr-2）和锆卡迪恩-705（Zr705）表面黏附的数据及分析。这些锆基材料与目前用于全髋关节和膝关节置换的材料相似。在此，我们研究了在振荡和静态暴露条件下微生物的临床菌株，以及它们黏附于具有不同氧化层厚度的锆表面的能力。我们使用X射线光电子能谱（XPS）、扫描电子显微镜（SEM）、活菌计数、内毒素测定和统计分析方法，并基于XPS数据建立了微生物黏附的预测模型。
