Rodrigues L R, Banat I M, van der Mei H C, Teixeira J A, Oliveira R
Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga, Portugal.
J Appl Microbiol. 2006 Mar;100(3):470-80. doi: 10.1111/j.1365-2672.2005.02826.x.
The effects and extent of adhesion of four different bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber with and without an adsorbed rhamnolipid biosurfactant layer obtained from Pseudomonasaeruginosa DS10-129 was studied.
The ability of rhamnolipid biosurfactant to inhibit adhesion of micro-organisms to silicone rubber was investigated in a parallel-plate flow chamber. The anti-adhesive activity of the biosurfactant at different concentrations was significant against all the strains and depended on the micro-organism tested. The results showed an effective reduction in the initial deposition rates, and the number of bacterial cells adhering after 4 h, for all micro-organisms tested at the 4 g l(-1) undiluted rhamnolipid solution. Maximum initial reduction of adhesion rate (an average of 66%) occurred for Streptococcus salivarius GB 24/9 and Candida tropicalis GB 9/9. The number of cells adhering after 4 h on silicone rubber conditioned with biosurfactant was reduced to 48% for Staphylococcus epidermidis GB 9/6, Strep. salivarius GB 24/9, Staphylococcus aureus GB 2/1 and C. tropicalis GB 9/9 in comparison to controls. Perfusing the flow chamber with biosurfactant containing solution followed by the passage of a liquid-air interface, to investigate detachment of micro-organisms adhering to silicone rubber, produced high detachment (96%) of adhered cells for all micro-organisms studied, except for Staph. aureus GB 2/1 (67%).
It is concluded that biosurfactant represent suitable compounds that should be considered in developing future strategies to prevent the microbial colonization of silicone rubber voice prostheses.
研究从植入的语音假体中分离出的四种不同细菌菌株和两种酵母菌株，在有无吸附了源自铜绿假单胞菌DS10 - 129的鼠李糖脂生物表面活性剂层的硅橡胶上的黏附效果及程度。
在平行平板流动腔中研究了鼠李糖脂生物表面活性剂抑制微生物黏附到硅橡胶上的能力。不同浓度生物表面活性剂的抗黏附活性对所有菌株均有显著作用，且取决于所测试的微生物。结果表明，在4 g l⁻¹未稀释的鼠李糖脂溶液中，所有测试微生物的初始沉积速率和4小时后黏附的细菌细胞数量均有效降低。唾液链球菌GB 24/9和热带假丝酵母GB 9/9的黏附率初始降低幅度最大（平均为66%）。与对照相比，在经生物表面活性剂处理的硅橡胶上，4小时后表皮葡萄球菌GB 9/6、唾液链球菌GB 24/9、金黄色葡萄球菌GB 2/1和热带假丝酵母GB 9/9的黏附细胞数量减少至48%。用含生物表面活性剂的溶液灌注流动腔，随后通过液 - 气界面，以研究黏附在硅橡胶上的微生物的脱离情况，结果显示，除金黄色葡萄球菌GB 2/1（67%）外, 所有研究的微生物的黏附细胞均有较高的脱离率（96%）。
得出的结论是，生物表面活性剂是合适的化合物，在制定未来预防硅橡胶语音假体微生物定植的策略时应予以考虑。
