Li Chunya
College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
Colloids Surf B Biointerfaces. 2006 Jul 1;50(2):147-51. doi: 10.1016/j.colsurfb.2006.05.004. Epub 2006 May 16.
A novel film electrode for the voltammetric determination of tyrosine has been constructed based on electropolymerization of L-serine on a glassy carbon electrode. Voltammetric behaviour of tyrosine on the poly-L-serine film electrode was investigated with cyclic voltammetry and linear sweep voltammetry, and electrochemical parameters were calculated from chronocoulometry. In optimal conditions, the oxidation peak current of tyrosine on the poly-L-serine film electrode was enhanced greatly. A sensitive oxidation peak at 0.90 V was employed to determine tyrosine. A linearity between the oxidation peak current and the tyrosine concentration was obtained in the range of 3.0 x 10(-7) to 1.0 x 10(-4) mol L(-1) with a detection limit of 1.0 x 10(-7)mol L(-1). The practical application of the film electrode in the determination of tyrosine in a commercial amino acid oral solution demonstrated that it has good selectivity and high sensitivity.
基于L-丝氨酸在玻碳电极上的电聚合作用，构建了一种用于伏安法测定酪氨酸的新型薄膜电极。采用循环伏安法和线性扫描伏安法研究了酪氨酸在聚-L-丝氨酸薄膜电极上的伏安行为，并通过计时库仑法计算了电化学参数。在最佳条件下，聚-L-丝氨酸薄膜电极上酪氨酸的氧化峰电流显著增强。利用0.90 V处的灵敏氧化峰测定酪氨酸。氧化峰电流与酪氨酸浓度在3.0×10⁻⁷至1.0×10⁻⁴ mol L⁻¹范围内呈线性关系，检测限为1.0×10⁻⁷ mol L⁻¹。该薄膜电极在商业氨基酸口服液中酪氨酸测定的实际应用表明，它具有良好的选择性和高灵敏度。
