Faculty of Science and Environment, "Dunărea de Jos" University of Galati, 800008 Galati, Romania.
Int J Mol Sci. 2021 Jul 14;22(14):7528. doi: 10.3390/ijms22147528.
Good health, of vital importance in order to carry out our daily routine, consists of both physical and mental health. Tyrosine (Tyr) deficiency as well as its excess are issues that can affect mental health and can generate disorders such as depression, anxiety, or stress. Tyr is the amino acid (AA) responsible for maintaining good mental health, and for this reason, the present research presents the development of new electrochemical sensors modified with polypyrrole (PPy) doped with different doping agents such as potassium hexacyanoferrate (II) (FeCN), sodium nitroprusside (NP), and sodium dodecyl sulfate (SDS) for a selective and sensitive detection of Tyr. The development of the sensors was carried out by chronoamperometry (CA) and the electrochemical characterization was carried out by cyclic voltammetry (CV). The detection limits (LOD) obtained with each modified sensor were 8.2 × 10 M in the case of PPy /FeCN-SPCE, 4.3 × 10 M in the case of PPy/NP-SPCE, and of 3.51 × 10 M in the case of PPy/SDS-SPCE, thus demonstrating a good sensitivity of these sensors detecting L-Tyr. The validation of sensors was carried out through quantification of L-Tyr from three pharmaceutical products by the standard addition method with recoveries in the range 99.92-103.97%. Thus, the sensors present adequate selectivity and can be used in the pharmaceutical and medical fields.
身体健康对于我们进行日常活动至关重要，它包括身体健康和心理健康。色氨酸（Tyr）缺乏和过量都是会影响心理健康的问题，并可能导致抑郁、焦虑或压力等疾病。Tyr 是维持良好心理健康的氨基酸（AA），因此，本研究提出了开发新型电化学传感器的方法，该传感器使用聚吡咯（PPy）修饰，掺杂不同的掺杂剂，如铁氰化钾（II）（FeCN）、硝普酸钠（NP）和十二烷基硫酸钠（SDS），用于 Tyr 的选择性和灵敏检测。传感器的开发通过计时安培法（CA）进行，电化学表征通过循环伏安法（CV）进行。每个修饰传感器的检测限（LOD）分别为：PPy/FeCN-SPCE 为 8.2×10-7 M，PPy/NP-SPCE 为 4.3×10-7 M，PPy/SDS-SPCE 为 3.51×10-7 M，这表明这些传感器对 L-Tyr 的检测具有良好的灵敏度。通过标准加入法对三种药物产品中的 L-Tyr 进行定量，回收率在 99.92-103.97%范围内，对传感器进行了验证。因此，这些传感器具有足够的选择性，可用于制药和医疗领域。
