Cooke Carole A, McGimpsey Stuart, Mahon Gerald, Best Richard M
Department of Ophthalmology and the Queen's University Belfast Ophthalmic Research Centre, Royal Victoria Hospital, Northern Ireland, UK.
Invest Ophthalmol Vis Sci. 2006 Jul;47(7):2985-9. doi: 10.1167/iovs.05-1275.
To demonstrate differences in human lens epithelial cell adhesion to different intraocular lens biomaterials in vitro and to determine whether these differences can be influenced by coating the intraocular lens surface with commercially available fibronectin.
A prospective laboratory-based study comparing human lens epithelial cell adhesion to silicone (n=18), polymethylmethacrylate (PMMA; n=18), and acrylic (n=18) intraocular lenses in vitro. The three types of intraocular lenses were then coated with fibronectin: silicone (n=6), PMMA (n=6), and acrylic (n=6). The main outcome measure was the mean number of lens epithelial cells attached to each lens type after 24 hours of incubation.
In the uncoated lens group, there was a significantly higher number of lens epithelial cells attached to the acrylic than to the silicone or PMMA lenses (P<0.001). Coating the lenses with fibronectin caused a significant increase in attachment of lens epithelial cells for all three lens types.
There was a significant difference in the degree of lens epithelial cell attachment to the various types of intraocular lenses in vitro. Cell attachment was more prominent in the acrylic lenses, but the fibronectin coating negated differences in lens type and caused a significant increase in cell attachment across all groups.
在体外证明人晶状体上皮细胞对不同人工晶状体生物材料的黏附差异，并确定用市售纤连蛋白包被人工晶状体表面是否会影响这些差异。
一项基于实验室的前瞻性研究，比较人晶状体上皮细胞在体外对硅胶（n = 18）、聚甲基丙烯酸甲酯（PMMA；n = 18）和丙烯酸酯（n = 18）人工晶状体的黏附情况。然后对这三种类型的人工晶状体用纤连蛋白进行包被：硅胶（n = 6）、PMMA（n = 6）和丙烯酸酯（n = 6）。主要观察指标是孵育24小时后附着于每种晶状体类型的晶状体上皮细胞的平均数。
在未包被的晶状体组中，附着于丙烯酸酯晶状体的晶状体上皮细胞数量显著高于硅胶或PMMA晶状体（P < 0.001）。用纤连蛋白包被晶状体导致所有三种晶状体类型的晶状体上皮细胞附着显著增加。
体外人晶状体上皮细胞对不同类型人工晶状体的黏附程度存在显著差异。细胞黏附在丙烯酸酯晶状体中更为显著，但纤连蛋白包被消除了晶状体类型的差异，并导致所有组的细胞黏附显著增加。
