Schroeder Andreas C, Schmidbauer Josef M, Sobke Astrid, Seitz Berthold, Ruprecht Klaus W, Herrmann Mathias
Department and Hospital of Ophthalmology, University of Saarland Hospital, Homburg/Saar, Germany.
J Cataract Refract Surg. 2008 Mar;34(3):497-504. doi: 10.1016/j.jcrs.2007.10.042.
To determine the effect of the modification of intraocular lens (IOL) surface properties on the adhesion of Staphylococcus epidermidis caused by fibronectin (FN) as the predominant proadhesive glycoprotein of the eye's initial foreign body reaction.
University of Saarland, Homburg/Saar, Germany.
Eleven IOL types were tested. The IOLs were of poly(methyl methacrylate), acrylate, or silicone. Some were surface modified with heparin or polysaccharide coating. The IOLs, unadsorbed or preadsorbed with fibronectin (FN), were incubated with [(3)H]-thymidine-labeled S epidermidis Rp62a, and the amount of adherent microorganisms was determined.
Attachment of S epidermidis adhesion to various types of IOLs, both unadsorbed and FN precoated, varied significantly. The attachment to highly adhesive IOLs was almost 4-fold greater than that to low-adhesive IOLs. Attachment to FN precoated IOLs was generally enhanced compared with attachment to unadsorbed IOLs. Heparin surface modification resulted in no or a modest reduction in bacterial adhesion compared with unmodified IOLs. Bacterial adhesion was highly statistically significantly less on IOLs with polysaccharide surface modification.
There was significant variability in S epidermidis adhesion to IOLs as a function of design, material, surface modification, and FN preadsorption. Application of the findings may foster new developments to further reduce the major complication in cataract surgery, infective endophthalmitis.
确定人工晶状体（IOL）表面特性的改变对表皮葡萄球菌黏附的影响，纤连蛋白（FN）是眼部初始异物反应中主要的促黏附糖蛋白。
德国萨尔州洪堡市萨尔兰大学。
测试了11种IOL类型。这些IOL由聚甲基丙烯酸甲酯、丙烯酸酯或硅酮制成。一些IOL进行了肝素或多糖涂层表面改性。将未吸附或预吸附有纤连蛋白（FN）的IOL与[³H] - 胸苷标记的表皮葡萄球菌Rp62a孵育，测定黏附微生物的数量。
表皮葡萄球菌对各种类型IOL（未吸附和FN预包被）的黏附存在显著差异。对高黏附性IOL的黏附几乎比对低黏附性IOL的黏附高4倍。与对未吸附IOL的黏附相比，对FN预包被IOL的黏附通常有所增强。与未改性IOL相比，肝素表面改性导致细菌黏附无明显降低或略有降低。多糖表面改性的IOL上细菌黏附在统计学上显著减少。
表皮葡萄球菌对IOL的黏附因设计、材料、表面改性和FN预吸附而存在显著差异。这些研究结果的应用可能会推动新的进展，以进一步减少白内障手术的主要并发症——感染性眼内炎。
