Soejima Hirofumi, Irie Atsushi, Fukunaga Takashi, Sugamura Koichi, Kojima Sunao, Sakamoto Tomohiro, Yoshimura Michihiro, Kishikawa Hideki, Nishimura Yasuharu, Ogawa Hisao
Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, Japan.
Circ J. 2006 Jul;70(7):851-6. doi: 10.1253/circj.70.851.
Plaque instability in patients with unstable angina (UA) is associated with stimulated CD4+ T cells, so the present study investigated whether there is a relationship among plaque instability, osteopontin and CD4+ T cells.
Peripheral blood mononuclear cells were collected from 51 consecutive patients with UA, 60 patients with stable angina (SA), and 39 patients with chest pain syndrome (CPS). Osteopontin-producing CD4+ T cells were quantified by flow cytometry. Plasma osteopontin levels (ng/ml) were measured by ELISA and were higher in patients with UA (792.0 +/- 316.7) than in those with SA (626.0 +/- 195.0, p < 0.005) or CPS (594.7 +/- 239.4, p < 0.005). The frequency (%) of osteopontin-producing CD4+ T cells was higher in patients with UA (26.7 +/- 13.3) than in those with SA (19.5 +/- 11.1, p < 0.05) or CPS (16.6 +/- 9.0, p < 0.005). Furthermore, the plasma osteopontin level correlated with the frequency of osteopontin-producing CD4+ T cells (r = 0.327, p = 0.0004), as did the high-sensitivity C-reactive protein level (r = 0.360, p = 0.0002).
The plasma osteopontin levels are elevated in patients with UA, accompanied by an increase in the number of osteopontin-production of circulating CD4+ T cells. Circulating CD4+ T cells may play a role through osteopontin in the pathophysiology of UA.
不稳定型心绞痛（UA）患者的斑块不稳定与活化的CD4+ T细胞有关，因此本研究调查了斑块不稳定、骨桥蛋白和CD4+ T细胞之间是否存在关联。
连续收集51例UA患者、60例稳定型心绞痛（SA）患者和39例胸痛综合征（CPS）患者的外周血单个核细胞。通过流式细胞术对产生骨桥蛋白的CD4+ T细胞进行定量。采用酶联免疫吸附测定法（ELISA）检测血浆骨桥蛋白水平（ng/ml），UA患者（792.0±316.7）高于SA患者（626.0±195.0，p<0.005）或CPS患者（594.7±239.4，p<0.005）。UA患者中产生骨桥蛋白的CD4+ T细胞频率（%）（26.7±13.3）高于SA患者（19.5±11.1，p<0.05）或CPS患者（16.6±9.0，p<0.005）。此外，血浆骨桥蛋白水平与产生骨桥蛋白的CD4+ T细胞频率相关（r = 0.327，p = 0.0004），高敏C反应蛋白水平也与之相关（r = 0.360，p = 0.0002）。
UA患者血浆骨桥蛋白水平升高，同时循环中产生骨桥蛋白的CD4+ T细胞数量增加。循环中的CD4+ T细胞可能通过骨桥蛋白在UA的病理生理过程中发挥作用。
