Donly B C, Tate W P
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Proc Biol Sci. 1991 Jun 22;244(1311):207-10. doi: 10.1098/rspb.1991.0072.
Normal translation of the gene for E. coli release factor 2 (RF-2) is characterized by a +1 frameshift event that occurs with 30-50% efficiency. Frameshifting on synthetic RF-2 mRNA by eukaryotic ribosomes has also been observed, even though they lack the capability to interact with the frameshift signal in the same manner as prokaryotic ribosomes. We have mutagenized the sequence of the RF-2 gene to eliminate the need for a frameshift, thereby allowing frameshifting efficiency to be measured by direct comparison of RF-2 production from the mutant with production from the wild-type. Measurements using this approach confirm that frameshifting by rabbit reticulocyte lysate ribosomes occurs at the frameshift region, but with a limited efficiency of approximately 0.4%.
大肠杆菌释放因子2（RF - 2）基因的正常翻译特征是发生+1移码事件，其效率为30 - 50%。即使真核核糖体缺乏与原核核糖体以相同方式与移码信号相互作用的能力，但在合成的RF - 2 mRNA上也观察到了真核核糖体的移码现象。我们对RF - 2基因序列进行了诱变，以消除移码的必要性，从而通过直接比较突变体与野生型的RF - 2产量来测量移码效率。使用这种方法进行的测量证实，兔网织红细胞裂解物核糖体的移码发生在移码区域，但效率有限，约为0.4%。
