大肠杆菌argI信使核糖核酸（mRNA）翻译过程中的核糖体移码错误。
A ribosomal frameshifting error during translation of the argI mRNA of Escherichia coli.
作者信息
Fu C, Parker J
机构信息
Department of Microbiology, Southern Illinois University, Carbondale 62901.
出版信息
Mol Gen Genet. 1994 May 25;243(4):434-41. doi: 10.1007/BF00280474.
Abstract
Using fusions between the Escherichia coli genes argI and lacZ, it has been demonstrated that ribosomal frameshifting occurs at a frequency of between 3% and 16% within the argI mRNA, soon after the initiation codon. The frameshift involves a phenylalanyl-tRNA shifting into the +1 frame at the sequence UUU-U/C. The shift does not occur if the in-frame phenylalanine codon UUU is replaced by UUC. The level of frameshifting is higher in dense cultures and is not dependent on phenylalanine starvation. In the wild-type argI gene this frameshifting event would be an error, leading to a truncated, non-functional protein. Therefore, it is unlike the numerous examples of required frameshifting events that have been described in other genes.
摘要
利用大肠杆菌基因argI和lacZ之间的融合，已证明核糖体移码在起始密码子后不久的argI mRNA内以3%至16%的频率发生。移码涉及苯丙氨酰-tRNA在UUU-U/C序列处移至+1读框。如果框内苯丙氨酸密码子UUU被UUC取代，则不会发生移码。移码水平在高密度培养物中更高，且不依赖于苯丙氨酸饥饿。在野生型argI基因中，这种移码事件将是一个错误，导致产生截短的、无功能的蛋白质。因此，它不同于其他基因中描述的众多必需移码事件的例子。
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