Bousbaa H, Fleury-Feith J
Faculté de Médecine, Université Paris XII, Créteil, France.
Am Rev Respir Dis. 1991 Sep;144(3 Pt 1):714-7. doi: 10.1164/ajrccm/144.3_Pt_1.714.
Histologic studies using the silver stain method have implicated pulmonary neuroendocrine cells (NEC) in asthma by demonstrating an increase in their number in the bronchi of guinea pigs actively sensitized with ovalbumin and 10 min after challenge. We verified the same data and completed them by a study of the long-standing effects of a challenge on NEC number in guinea pig bronchi. Actively sensitized animals were killed 2, 6, 24, 48, 72, and 144 h after being challenged by an aerosolized solution of ovalbumin. This study was completed by the evaluation of eosinophilic infiltration of bronchi to test the recently proposed hypothesis of the possible eosinophil recruitment by NEC product. Our results confirmed the increase in NEC number in the bronchial wall after sensitization. Originally we demonstrated that, 24 h after challenge, the NEC number decreased significantly, compared to sensitized only animals, suggesting possible product release. Eosinophilic migration was observed in sensitized animals and, more importantly, in all sensitized plus challenged animals. We suggest that bronchial NEC may play a role in immunoallergic events that take place in the lung after challenge, probably by releasing mediators that may influence, among other effects, eosinophilic recruitment.
使用银染法的组织学研究表明，在经卵清蛋白主动致敏的豚鼠支气管中，肺神经内分泌细胞（NEC）数量增加，这表明NEC与哮喘有关。在用卵清蛋白雾化溶液激发后10分钟，我们验证了相同的数据，并通过研究激发对豚鼠支气管中NEC数量的长期影响来完善这些数据。主动致敏的动物在受到卵清蛋白雾化溶液激发后2、6、24、48、72和144小时被处死。通过评估支气管嗜酸性粒细胞浸润来完成本研究，以检验最近提出的关于NEC产物可能募集嗜酸性粒细胞的假说。我们的结果证实了致敏后支气管壁中NEC数量的增加。最初我们证明，与仅致敏的动物相比，激发后24小时，NEC数量显著减少，提示可能有产物释放。在致敏动物中观察到了嗜酸性粒细胞迁移，更重要的是，在所有致敏加激发的动物中都观察到了嗜酸性粒细胞迁移。我们认为，支气管NEC可能在激发后肺中发生的免疫过敏事件中起作用，可能是通过释放可能影响嗜酸性粒细胞募集等效应的介质来实现的。
