通过螯合作用分离肾母细胞瘤抗原

Isolation of Wilms' tumor antigens by chelation.

作者信息

Beierle J W, Wise K S, Trump G N, Allerton S E

出版信息

Clin Chim Acta. 1975 Jun 20;61(3):411-4. doi: 10.1016/0009-8981(75)90433-7.

Abstract

A procedure for ethylenediaminetetraacetate extraction of minced Wilm's tumor was assessed as a method for isolating Wilm's tumor antigens. An antigen was detected by immunodiffusion using an adsorbed antiserum to this extract. This antigen was also found in ethylenediaminetetraacetate extracts of in vitro cultures of nephroblastoma cells.

摘要

评估了一种用于乙二胺四乙酸提取肾母细胞瘤碎块的程序，作为分离肾母细胞瘤抗原的一种方法。使用针对该提取物的吸附抗血清，通过免疫扩散检测到一种抗原。在肾母细胞瘤细胞体外培养物的乙二胺四乙酸提取物中也发现了这种抗原。