Kalita Michał, Stepkowski Tomasz, Łotocka Barbara, Małek Wanda
Department of General Microbiology, M. Curie-Skłodowska University, 19 Akademicka St., 20-033 Lublin, Poland.
Arch Microbiol. 2006 Aug;186(2):87-97. doi: 10.1007/s00203-006-0124-6. Epub 2006 Jun 21.
Pairwise comparisons of Genista tinctoria (dyer's weed) rhizobium nodA, nodC, and nodZ gene sequences to those available in databanks revealed their highest sequence identities to nodulation loci of Bradyrhizobium sp. (Lupinus) strains and rhizobia from other genistoid legumes. On phylogenetic trees, genistoid microsymbionts were grouped together in monophyletic clusters, which suggested that their nodulation genes evolved from a common ancestor. G. tinctoria nodulators formed symbioses not only with the native host, but also with other plants of Genisteae tribe such as: Lupinus luteus, Sarothamnus scoparius, and Chamaecytisus ratisbonensis, and they were classified as the genistoid cross-inoculation group. The dyer's weed root nodules were designated as indeterminate with apical meristem consisting of infected and uninfected cells.
将染料木根瘤菌的nodA、nodC和nodZ基因序列与数据库中已有的序列进行两两比较，结果显示它们与慢生根瘤菌属（羽扇豆）菌株以及其他染料木类豆科植物的根瘤菌的结瘤基因座具有最高的序列同一性。在系统发育树上，染料木类微共生体聚集在单系类群中，这表明它们的结瘤基因起源于一个共同的祖先。染料木的根瘤菌不仅与本地宿主形成共生关系，还与染料木族的其他植物形成共生关系，如：黄羽扇豆、帚石南和拉蒂斯邦山羊豆，它们被归类为染料木类交叉接种组。染料木的根瘤被认定为顶端分生组织由感染细胞和未感染细胞组成的不定型根瘤。
