Nadol'nik L I, Vinogradov V V
Vopr Med Khim. 1991 Mar-Apr;37(2):34-7.
High performance liquid chromatography was used to determine corticosterone concentration and transcortin binding capacity (TBC) in blood plasma of rats with bilateral adrenalectomy, before and after removal of endogenous hormones within 7 days (0.4, 0.2, 1, 2, 3, 4, 5, 7 days). During the first day TBC significantly decreased. However, concentration of protein binding sites subsequently elevated exceeding 2-fold the level of control values within the 7th day of the experiment. Concentration of 14C-immunoreactive transcortin in rat liver cytosol did not differ from control levels on the 5th day after the adrenalectomy. The adrenalectomy did not affect the transcortin affinity constant for corticosterone. These data suggest that the increased rate of blood plasma TBC was not related to activation of liver specific protein biosynthesis.
采用高效液相色谱法测定双侧肾上腺切除大鼠血浆中皮质酮浓度及皮质素结合容量（TBC），于切除内源性激素后的7天内（0.4、0.2、1、2、3、4、5、7天），分别在术前及术后进行检测。在第一天，TBC显著降低。然而，在实验的第7天，蛋白质结合位点的浓度随后升高，超过对照值水平的2倍。肾上腺切除术后第5天，大鼠肝细胞溶胶中14C免疫反应性皮质素的浓度与对照水平无差异。肾上腺切除术不影响皮质素对皮质酮的亲和常数。这些数据表明，血浆TBC升高的速率与肝脏特异性蛋白质生物合成的激活无关。
