Kinnunen A, Panula P
Department of Anatomy, University of Helsinki, Finland.
Agents Actions. 1991 May;33(1-2):108-11. doi: 10.1007/BF01993140.
The distribution of histamine and tyrosine hydroxylase in fetal rat brain was investigated immunocytochemically in order to determine possible colocalization of these two substances. Embryonic rat brains were fixed with carbodiimide and processed for immunofluorescence studies with antisera against histamine and tyrosine hydroxylase either in the same sections or in consecutive sections. Histamine and tyrosine hydroxylase showed no colocalisation in the developing rat brain. However, fibre networks immunoreactive for histamine and tyrosine hydroxylase were often found in the same areas. The results of the study suggest that the catecholaminergic and histaminergic neurones develop separately in the rat brain. Based on the location of developing histamine-immunoreactive neurones, a more intimate relationship between histamine- and serotonin-containing neurones in the developing rat brain is plausible.
为了确定这两种物质是否可能共定位，采用免疫细胞化学方法研究了组胺和酪氨酸羟化酶在胎鼠脑中的分布。用碳二亚胺固定胚胎大鼠脑，并使用抗组胺和酪氨酸羟化酶的抗血清在同一切片或连续切片中进行免疫荧光研究。组胺和酪氨酸羟化酶在发育中的大鼠脑中未显示共定位。然而，对组胺和酪氨酸羟化酶呈免疫反应的纤维网络经常出现在同一区域。研究结果表明，大鼠脑中儿茶酚胺能神经元和组胺能神经元是分别发育的。根据发育中组胺免疫反应性神经元的位置，发育中的大鼠脑中组胺能神经元和5-羟色胺能神经元之间存在更密切的关系是合理的。
