Päivärinta H, Häppölä O, Joh T H, Steinbusch H, Watanabe T, Panula P
Department of Anatomy, University of Helsinki, Finland.
Histochem J. 1987 Jun-Jul;19(6-7):319-26. doi: 10.1007/BF01680447.
The coexistence of histamine, histidine decarboxylase (the enzyme synthesizing histamine), 5-hydroxytryptamine and tyrosine hydroxylase (the rate-limiting enzyme in catecholamine synthesis), was studied in the rat superior cervical ganglion with the indirect immunofluorescence method. Possible colocalization was examined by staining consecutive sections with two different antibodies, or alternatively in the same section by eluting the first antibody with a mild solution containing potassium permanganate and sulphuric acid, and by staining the same section with another antibody. It was shown that tyrosine hydroxylase immunoreactivity was found both in large principal nerve cells and in small cells, which on the basis of their size and high nucleus-cytoplasm ratio corresponded to small intensely fluorescent (SIF) cells. Histamine, histidine decarboxylase and 5-hydroxytryptamine immunoreactivities were observed only in SIF cells. Those SIF cells which were immunoreactive for histamine, histidine decarboxylase or 5-hydroxytryptamine also contained tyrosine hydroxylase immunoreactivity. On the other hand, all tyrosine hydroxylase-immunoreactive SIF cells were also immunoreactive for histidine decarboxylase or 5-hydroxytryptamine. Some of the SIF cells, which were non-reactive for histamine, were immunoreactive for tyrosine hydroxylase.
采用间接免疫荧光法研究了大鼠颈上神经节中组胺、组胺酸脱羧酶（合成组胺的酶）、5-羟色胺和酪氨酸羟化酶（儿茶酚胺合成中的限速酶）的共存情况。通过用两种不同抗体对连续切片进行染色来检测可能的共定位，或者通过用含有高锰酸钾和硫酸的温和溶液洗脱第一种抗体，然后用另一种抗体对同一切片进行染色，在同一切片中进行检测。结果表明，酪氨酸羟化酶免疫反应性在大型主神经细胞和小型细胞中均有发现，根据其大小和高核质比，这些小型细胞对应于小而强荧光（SIF）细胞。组胺、组胺酸脱羧酶和5-羟色胺免疫反应性仅在SIF细胞中观察到。那些对组胺、组胺酸脱羧酶或5-羟色胺呈免疫反应性的SIF细胞也含有酪氨酸羟化酶免疫反应性。另一方面，所有对酪氨酸羟化酶呈免疫反应性的SIF细胞对组胺酸脱羧酶或5-羟色胺也呈免疫反应性。一些对组胺无反应的SIF细胞对酪氨酸羟化酶呈免疫反应性。
