Baquet A, Maisin L, Hue L
Hormone and Metabolic Research Unit, University of Louvain Medical School, Brussels, Belgium.
Biochem J. 1991 Sep 15;278 ( Pt 3)(Pt 3):887-90. doi: 10.1042/bj2780887.
Incubation of hepatocytes in conditions known to increase their volume, i.e. with amino acids or in hypo-osmotic media, resulted in the parallel activation of glycogen synthase and acetyl-CoA carboxylase. The activation of both enzymes by glutamine was antagonized by the addition of raffinose to prevent cell swelling, or by glucagon and microcystin. The findings are consistent with the involvement of a common mechanism for the activation of the two enzymes.
在已知会增加肝细胞体积的条件下培养肝细胞，即使用氨基酸或在低渗培养基中培养，会导致糖原合酶和乙酰辅酶A羧化酶同时被激活。谷氨酰胺对这两种酶的激活作用可通过添加棉子糖以防止细胞肿胀，或通过胰高血糖素和微囊藻毒素来拮抗。这些发现与这两种酶激活的共同机制的参与是一致的。
