Nielsen Elise R, Eskildsen-Helmond Yvonne E G, Rattan Suresh I S
Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark.
Ann N Y Acad Sci. 2006 May;1067:343-8. doi: 10.1196/annals.1354.048.
Adult human skin fibroblasts were exposed repeatedly to 41 degrees C or 42 degrees C heat shock (HS) for 1 h twice a week during serial passaging throughout their replicative life span. On the basis of longevity curves, cell size, and morphology, we observed that repeated mild heat shock (RMHS) at 41, degrees C had strong anti-aging hormetic effects, including 20% extension of cellular longevity. The basal levels of the MAP kinases JNK1, JNK2, and p38 increased during serial passaging, while that of ERK2 decreased. RMHS further exaggerated these effects, which suggests that age-related changes in MAP kinases may be an adaptive response for better cell survival.
在人成纤维细胞的整个复制寿命期间进行传代培养时,每周两次将其反复暴露于41℃或42℃热休克(HS)1小时。根据寿命曲线、细胞大小和形态,我们观察到41℃的反复轻度热休克(RMHS)具有强大的抗衰老应激效应,包括细胞寿命延长20%。在传代培养过程中,丝裂原活化蛋白激酶JNK1、JNK2和p38的基础水平升高,而ERK2的基础水平降低。RMHS进一步加剧了这些效应,这表明丝裂原活化蛋白激酶的年龄相关变化可能是一种促进细胞更好存活的适应性反应。