Kwon Soon-Hyo, Na Jung-Im, Huh Chang-Hun, Park Kyoung-Chan
Department of Dermatology, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, Seoul, Korea.
Department of Dermatology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea.
Ann Dermatol. 2021 Dec;33(6):522-530. doi: 10.5021/ad.2021.33.6.522. Epub 2021 Nov 4.
Melasma shows characteristic histological features of photoaged skin.
We evaluated the effect of dermal rejuvenation using a temperature-controlled continuous non-invasive radiofrequency (RF) device on melasma.
Continuous skin heating at the temperature of 43°C for 20 minutes was performed in ten subjects with melasma who underwent 3 tri-weekly RF sessions. Pigmentation was evaluated with Mexameter® and investigator's global assessment (IGA). Immunohistochemical staining and image analysis was performed to evaluate biopsies from melasma skin before and after the treatment.
The lesional melanin index was decreased by 13.7% at week 9. IGA score was improved from 3.50 at baseline to 2.95 at week 9. No significant adverse event was reported. Histologic analysis revealed reduced melanin and increased collagen density and thickness. The expression of procollagen-1 and type IV collagen was increased after the treatment. The number of p16-positive senescent fibroblasts was reduced after the treatment, while the expression of heat shock protein 70 and 90 was increased. Stromal derived factor-1, a senescence-associated anti-melanogenic factor secreted from the fibroblasts, was up-regulated after the treatment, while the level of c-kit was not changed.
Thermal skin stimulation by the temperature-controlled continuous RF device improved melasma through dermal rejuvenation.
黄褐斑表现出光老化皮肤的特征性组织学特征。
我们评估了使用温度控制的连续非侵入性射频（RF）设备进行皮肤年轻化对黄褐斑的影响。
对10名患有黄褐斑的受试者进行连续皮肤加热，温度为43°C，持续20分钟，每周进行3次，共进行3周。使用Mexameter®和研究者整体评估（IGA）评估色素沉着情况。对治疗前后的黄褐斑皮肤活检组织进行免疫组织化学染色和图像分析。
在第9周时，皮损黑色素指数下降了13.7%。IGA评分从基线时的3.50提高到第9周时的2.95。未报告明显不良事件。组织学分析显示黑色素减少，胶原蛋白密度和厚度增加。治疗后I型前胶原和IV型胶原的表达增加。治疗后p16阳性衰老成纤维细胞数量减少，而热休克蛋白70和90的表达增加。成纤维细胞分泌的衰老相关抗黑素生成因子基质细胞衍生因子-1在治疗后上调，而c-kit水平未改变。
温度控制的连续射频设备进行的皮肤热刺激通过皮肤年轻化改善了黄褐斑。
