Dasgupta M K, Kowalewska-Grochowska K, Larabie M, Costerton J W
University of Alberta, Canada.
Adv Perit Dial. 1991;7:165-8.
To study the significance of biofilm bacteria in CAPD peritonitis, in a preliminary study, we compared the growth of planktonic and biofilm bacteria by parallel cultures of the same PD effluent in 14 patients with CAPD-associated peritonitis. Planktonic bacteria were isolated by routine microbiologic culture techniques and biofilm bacteria by the use of a modified Robbins' device (MRD). Antibiotic sensitivity or MIC's of bacterial strains isolated by the two techniques were compared. This comparison of the IC identified a subgroup of patients who developed recurrent antibiotic resistant biofilm-induced peritonitis and their peritoneal catheters were subsequently removed.
为研究生物膜细菌在持续性非卧床腹膜透析（CAPD）相关性腹膜炎中的意义，在一项初步研究中，我们通过对14例CAPD相关性腹膜炎患者的相同腹膜透析流出液进行平行培养，比较了浮游细菌和生物膜细菌的生长情况。浮游细菌采用常规微生物培养技术分离，生物膜细菌则通过使用改良的Robbins装置（MRD）进行分离。比较了通过这两种技术分离出的细菌菌株的抗生素敏感性或最低抑菌浓度（MIC）。这种比较确定了一组发生复发性抗生素耐药性生物膜诱导性腹膜炎的患者亚组，随后移除了他们的腹膜导管。
