Yang Sung, Undar Akif, Zahn Jeffrey D
205, Hallowell, Department of Bioengineering, The Pennsylvania State University, University Park, PA, USA.
Lab Chip. 2006 Jul;6(7):871-80. doi: 10.1039/b516401j. Epub 2006 Apr 19.
A microfluidic device for continuous, real time blood plasma separation is introduced. The principle of the blood plasma separation from blood cells is supported by the Zweifach-Fung effect and was experimentally demonstrated using simple microchannels. The blood plasma separation device is composed of a blood inlet, a bifurcating region which leads to a purified plasma outlet, and a concentrated blood cell outlet. It was designed to separate blood plasma from an initial blood sample of up to 45% inlet hematocrit (volume percentage of cells). The microfluidic network was designed using an analogous electrical circuit, as well as analytical and numerical studies. The functionality of this device was demonstrated using defibrinated sheep blood. During 30 minutes of continuous blood infusion through the device, all the erythrocytes (red blood cells) traveled through the device toward the concentrated blood outlet while only the plasma was separated at the bifurcating regions and flowed towards the plasma outlet. The device has been operated continuously without any clogging or hemolysis of cells. The experimentally determined plasma selectivity with respect to blood hematocrit level was almost 100% regardless of the inlet hematocrit. The total plasma separation volume percent varied from 15% to 25% with increasing inlet hematocrit. Due to the device's simple structure and control mechanism, this microdevice is expected to be used for highly efficient continuous, real time cell-free blood plasma separation from blood samples for use in lab on a chip applications.
介绍了一种用于连续、实时分离血浆的微流控装置。血浆与血细胞分离的原理基于 Zweifach-Fung 效应，并通过简单的微通道进行了实验验证。该血浆分离装置由一个血液入口、一个通向纯化血浆出口的分叉区域和一个浓缩血细胞出口组成。它被设计用于从初始血细胞比容（细胞体积百分比）高达 45% 的血液样本中分离血浆。微流控网络采用类似的电路进行设计，并进行了分析和数值研究。使用去纤维蛋白羊血对该装置的功能进行了演示。在通过该装置连续输注血液 30 分钟的过程中，所有红细胞都通过该装置流向浓缩血液出口，而只有血浆在分叉区域被分离并流向血浆出口。该装置连续运行，没有任何细胞堵塞或溶血现象。无论入口血细胞比容如何，实验测定的血浆相对于血液血细胞比容水平的选择性几乎为 100%。随着入口血细胞比容的增加，血浆总分离体积百分比从 15% 变化到 25%。由于该装置结构简单且控制机制简便，预计这种微型装置将用于从血液样本中高效连续、实时地分离无细胞血浆，用于芯片实验室应用。
