Zhao Hongping, Wu Buling, Wu Hong, Su Lingyun, Pang Jianliang, Yang Tiehong, Liu Yanli
Department of Operative Dentistry and Endodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, P.R. China.
Biotechnol Lett. 2006 Aug;28(16):1299-304. doi: 10.1007/s10529-006-9086-7. Epub 2006 Jun 28.
Recombinant Streptococcus mutans glucan-binding protein D (rGbpD) was incorporated into poly(lactic-co-glycolic acid) (PLGA) microspheres which then were surface-coated with chitosan. The microspheres, with a mean diameter of ca. 1.8 microm, were intranasally administered in rats. There were elevated salivary immunoglobulin A and serum immunoglobulin G antibody responses to rGbpD, as well as lower molar caries scores in immunized animals as compared to sham immunized ones. The chitosan-coated PLGA microspheres are thus potentially useful for antigen delivery in dental caries vaccination.
