Dunlop J, Fear A, Griffiths R
Department of Biochemistry and Microbiology, University of St. Andrews, Fife, Scotland, UK.
Neuroreport. 1991 Jul;2(7):377-9. doi: 10.1097/00001756-199107000-00005.
In an attempt to investigate the apparent absolute selectivity of the synaptic vesicle L-glutamate carrier, L- and D-enantiomers of excitatory sulphur-containing amino acid (SAA) transmitter candidates (which are close structural analogues of L-glutamate) were tested for their capacity to compete for vesicular L-[3H]-glutamate uptake. All SAAs inhibited, to varying degrees (52-86%), the vesicular uptake of L-[3H]-glutamate. A similar level of inhibition was exerted by SAAs with either a shorter or equal carbon chain length to L-glutamate. Moreover, inhibition was stereospecific in favour of the D-enantiomers. These studies indicate an appreciable interaction of the SAAs with the recognition site of the vesicular L-glutamate carrier. Further investigations are required to establish the substrate potential of the SAAs.
为了研究突触囊泡L-谷氨酸载体明显的绝对选择性，对兴奋性含硫氨基酸（SAA）递质候选物的L-和D-对映体（它们是L-谷氨酸的紧密结构类似物）竞争囊泡L-[3H]-谷氨酸摄取的能力进行了测试。所有SAA均不同程度地（52%-86%）抑制了L-[3H]-谷氨酸的囊泡摄取。与L-谷氨酸碳链长度较短或相等的SAA产生了相似程度的抑制作用。此外，抑制作用具有立体特异性，有利于D-对映体。这些研究表明SAA与囊泡L-谷氨酸载体的识别位点有明显的相互作用。需要进一步研究来确定SAA的底物潜力。
